- ¥1380 - 2200
- 康朗生物
- kl-4470R
- 中国/美国/德国
- 2026年02月02日
- KLH conjugated synthetic peptide derived from human ZP4:301-400/540 <Extracellular>
- Rabbit
- Human, Mouse, Rat, Horse,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海康朗生物科技有限公司
- 库存：
大量
- 目录编号：
kl-4470R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
12个月
- 抗体英文名：
Zona pellucida glycoprotein 4 antibody
- 抗体名：
卵透明带糖蛋白4抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Horse,
- 免疫原：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500
- 亚型：
IgG
- 形态：
冻干粉或液体
- 应用范围：
KLH conjugated synthetic peptide derived from human ZP4:301-400/540 <Extracellular>
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
100ul 200ul
| 中文名称 | 卵透明带糖蛋白4抗体 |
| 别 名 | Processed zona pellucida sperm-binding protein 4; ZBP; Zona pellucida B protein; Zona pellucida glycoprotein 4; Zona pellucida protein B; Zona pellucida sperm binding protein 4; Zp-4; ZP1; ZP4; ZP4_HUMAN; ZPB. |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 发育生物学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Horse, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 57kDa |
| 细胞定位 | 细胞膜 分泌型蛋白 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZP4:301-400/540 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4. [provided by RefSeq, Jul 2008] Function: The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP4 may act as a sperm receptor. Subcellular Location: Secreted > extracellular space > extracellular matrix and Cell membrane. Tissue Specificity: Oocytes. Post-translational modifications: Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida. Similarity: Belongs to the ZP domain family. ZPB subfamily. Contains 1 P-type (trefoil) domain. Contains 1 ZP domain. SWISS: Q12836 Gene ID: 57829 Database links: Entrez Gene: 57829 Human Omim: 613514 Human SwissProt: Q12836 Human Unigene: 136241 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 |  Sample: Uterus(Mouse)Lysate at 40 ug Primary: Anti-Zonapellucida glycoprotein 4(bs-4470R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 57kD Observed band size: 57kD  Sample: Ovary (Mouse)Lysate at 40 ug Primary: Anti-Zonapellucida glycoprotein 4(bs-4470R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 57kD Observed band size: 57kD |
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文献和实验The zona pellucida is an extracellular glycoprotein coat that surrounds the oocyte and, in many mammals, provides the first barrier sperm must penetrate for fertilization to occur. The molecular interactions between the sperm and zona
Aggregation of ES Cells and Eight Cell Stage Embryos
(lubricated) wells. When we made wells on dry plates the embryos always died. 5.Make 5-6 wells in each drop and then return the plate to the incubator. Removal of the Zona pellucida from the embryo. - After recovering 2.5 day mouse embryos (see Flushing
【申请上传】Current Protocols in Immunology
Autoimmunity Unit 15.16 The Day-3 Thymectomy Model for Induction of Multiple Organ-Specific Autoimmune Diseases Unit 15.17 Autoimmune Ovarian Disease Induced by Immunization with Zona Pellucida (ZP3 Peptide Unit 15.18 Animal Models of Airway Sensitization
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