Phospho-Caspase-9 (Thr125) ant

ibody

价格¥1580

品牌康朗生物
货号kl-3082R
产地中国/美国/德国
更新日期2025年07月10日

应用范围WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=0.2μg /test IF=1:100-500
宿主Rabbit
适应物种Human, Mouse, Rat,

上海康朗生物科技有限公司

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供应商：
上海康朗生物科技有限公司
库存：
大量
目录编号：
kl-3082R
克隆性：
多克隆
抗原来源：
Rabbit
保质期：
12个月
抗体英文名：
Phospho-Caspase-9 (Thr125) antibody
抗体名：
磷酸化半胱氨酸蛋白酶9抗体
宿主：
Rabbit
适应物种：
Human, Mouse, Rat,
免疫原：
KLH conjugated Synthesised phosphopeptide derived from human Caspase-9 around the phosphorylation site of Thr125:PE(p-T)PR
亚型：
IgG
形态：
冻干粉或液体
应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=0.2μg /test IF=1:100-500
浓度：
1mg/ml
保存条件：
-20 °C
规格：
100ul

Phospho-Caspase-9 (Thr125) antibody

中文名称	磷酸化半胱氨酸蛋白酶9抗体
别名	APAF 3; APAF3; Apoptosis related cysteine peptidase; Apoptotic protease activating factor 3; Apoptotic protease Mch 6; CASP 9; CASP9; Caspase 9 Dominant Negative; Caspase 9 precursor; Caspase 9c; Caspase9; EC 3.4.22.; ICE LAP6; ICE like apoptotic protease 6; MCH6 antibody RNCASP9; CASP9_HUMAN.
规格价格	100ul/1580元购买大包装/询价
说明书	100ul
产品类型	磷酸化抗体
研究领域	细胞凋亡
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=0.2μg /test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	35kDa
细胞定位	细胞浆
性状	Lyophilized or Liquid
浓度	1mg/ml
免疫原	KLH conjugated Synthesised phosphopeptide derived from human Caspase-9 around the phosphorylation site of Thr125:PE(p-T)PR
亚型	IgG
纯化方法	affinity purified by Protein A
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013] Function: Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP). Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts with the inhibitors BIRC2, BIRC4, BIRC5 and BIRC7. Interacts (inactive form) with EFHD2. Interacts with HAX1. Tissue Specificity: Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. Post-translational modifications: Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Similarity: Belongs to the peptidase C14A family. Contains 1 CARD domain. SWISS: P55211 Gene ID: 842 Database links: Entrez Gene: 842 Human Entrez Gene: 12371 Mouse Entrez Gene: 58918 Rat Omim: 602234 Human SwissProt: P55211 Human SwissProt: Q4FJK5 Mouse SwissProt: Q920G4 Rat Unigene: 329502 Human Unigene: 88829 Mouse Unigene: 32199 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Caspase-9半胱氨酸蛋白酶家族成员之一，又称ICE-Lap6（ICE Like apoptotease 6）参与细胞凋亡过程和细胞因子的加工过程，在许多胚胎和成人组织中都有分布。此抗体主要用于肿瘤凋亡的研究。
产品图片	Sample: Brain(Rat)Lysate at 40 ug Primary: Anti-p-Caspase-9 (Thr125)(bs-3082R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 35kD Observed band size: 35kD Sample:H9C2 (Rat)cell Lysate at 40 ug Primary: Anti-p-Caspase-9 (Thr125)(bs-3082R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 35kD Observed band size: 35kD Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (P-caspase-9(Thr125)) Polyclonal Antibody, Unconjugated (bs-3082R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (P-caspase-9(Thr125)) Polyclonal Antibody, Unconjugated (bs-3082R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Caspase-9 (Thr125)) Polyclonal Antibody, Unconjugated (bs-3082R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Blank control (blue line): Hela (fixed with 70% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody (green line): Rabbit Anti-Phospho-Caspase-9 (Thr125) antibody (bs-3082R), Dilution: 0.2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC, Dilution: 1μg /test.