手机验证
上海联迈生物工程有限公司
大量
LM-0527R
多克隆
Rabbit
1年
CD10
CD10抗体
Rabbit
Human, Mouse, Rat,
KLH conjugated synthetic peptide derived from human CD10:601-600/750 <Extracellular>
IgG
Lyophilized or Liquid
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3μg /test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
1mg/ml
Store at -20 °C
50ul 100ul 200ul
英文名称 | CD10 |
中文名称 | CD10抗体 |
别 名 | Atriopeptidase; CALLA; CD 10; CD10; Common acute lymphocytic leukemia antigen; DKFZp686O16152; EC 3.4.24.11; Enkephalinase; EPN; gp100; Membrane metallo endopeptidase; Membrane metalloendopeptidase; Membrane metalloendopeptidase neutral endopeptidase enkephalinase; Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10; Membrane metalloendopeptidase variant 1; Membrane metalloendopeptidase variant 2; MGC126681; MGC126707; MME; NEP; Neprilysin; Neutral endopeptidase 24.11; Neutral endopeptidase; NEP_HUMAN; Neprilysin; Skin fibroblast elastase; SFE. |
规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
说 明 书 | 50ul 100ul 200ul |
研究领域 | 肿瘤 细胞生物 免疫学 干细胞 细胞表面分子 细胞类型标志物 淋巴细胞 b-淋巴细胞 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human, Mouse, Rat, |
产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=3μg /test ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
分 子 量 | 82kDa |
细胞定位 | 细胞膜 |
性 状 | Lyophilized or Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human CD10:601-600/750 <Extracellular> |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
PubMed | PubMed |
产品介绍 | background: This gene encodes a common acute lymphocytic leukemia antigen that is an important cell surface marker in the diagnosis of human acute lymphocytic leukemia (ALL). This protein is present on leukemic cells of pre-B phenotype, which represent 85% of cases of ALL. This protein is not restricted to leukemic cells, however, and is found on a variety of normal tissues. It is a glycoprotein that is particularly abundant in kidney, where it is present on the brush border of proximal tubules and on glomerular epithelium. The protein is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. This gene, which encodes a 100-kD type II transmembrane glycoprotein, exists in a single copy of greater than 45 kb. The 5' untranslated region of this gene is alternatively spliced, resulting in four separate mRNA transcripts. The coding region is not affected by alternative splicing. Function: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers. Subcellular Location: Cell membrane; Single-pass type II membrane protein. Post-translational modifications: Myristoylation is a determinant of membrane targeting. Glycosylation at Asn-628 is necessary both for surface expression and neutral endopeptidase activity. Similarity: Belongs to the peptidase M13 family. SWISS: P08473 Gene ID: 4311 Database links: Entrez Gene: 4311 Human Entrez Gene: 17380 Mouse Entrez Gene: 24590 Rat Omim: 120520 Human SwissProt: P08473 Human SwissProt: Q61391 Mouse SwissProt: P07861 Rat Unigene: 307734 Human Unigene: 296022 Mouse Unigene: 33598 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. CD10即共同型急性淋巴细胞白血病抗原,是II型跨膜糖蛋白.CD10表达于早期B淋巴细胞,某些粒性白细胞,骨髓基质细胞,部分上皮组织及其起源的肿瘤细胞中也有表达.主要应用于某些恶性淋巴瘤和白血病的分型.CD10是一种94kD的锌依赖性细胞膜金属结合蛋白,参与细胞神经肽和激素肽的后分泌过程。它广泛地分布于肾、肝、小肠、胎盘、脉络膜丛、脑腺、腺皮质和白细胞,CD10是生发中心细胞及所致淋巴瘤的标志物。 |
产品图片 | Sample: Raji(Human) Cell Lysate at 30 ug Primary: Anti-CD10 (bs-0527R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 82 kD Observed band size: 82 kD Blank control: A549 (blue). Primary Antibody:Rabbit Anti- CD10 antibody(bs-0527R), Dilution: 3μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (bs-0527R, 3μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed. |
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