- ¥780 - 2200
- LMAI Bio
- LM-0170R
- 进口/国产
- 2025年07月16日
- ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
- Rabbit
- Human, Mouse, Rat, Pig, Horse,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 目录编号：
LM-0170R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
VEGFR1
- 抗体名：
血管内皮生长因子受体1抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Pig, Horse,
- 免疫原：
KLH conjugated synthetic peptide derived from mouae VEGFR1:1162-1260/1333 <Cytoplasmic>
- 亚型：
IgG
- 形态：
Lyophilized or Liquid
- 应用范围：
ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
- 浓度：
1mg/ml
- 保存条件：
Store at -20 °C
- 规格：
50ul 100ul 200ul
VEGFR1血管内皮生长因子受体1抗体
| 英文名称 | VEGFR1 |
| 中文名称 | 血管内皮生长因子受体1抗体 |
| 别 名 | VEGF-R1; VEGFR-1; VEGF Receptor 1; FLT-1; vascular permeability factor receptor; vascular endothelial growth factor receptor; vascular endothelial growth factor receptor-1; fms-related tyrosine kinase 1; vascular endothelial growth factor/vascular permeability factor receptor; AI323757; FLT; FLT1; sFlt1; VGFR1_HUMAN; VGFR1_MOUSE. |
 |
Specific References (3) | bs-0170R has been referenced in 3 publications. [IF=2.38] Cheng, Gangwei, et al. "Direct Effects of Bevacizumab on Rat Conjunctival Fibroblast." Cell Biochemistry and Biophysics: 1-6. Rat. PubMed:25656769 [IF=2.83] Liu, Huayan, et al. "Paeoniflorin attenuates Aβ 1-42-induced inflammation and chemotaxis of microglia in vitro and inhibits NF-κB-and VEGF/Flt-1 signaling pathways." Brain Research (2015). WB ; Rat. PubMed:26049130 [IF=2.35] Liu, Yanhua, et al. "RUNX3 modulates hypoxia-induced endothelial-to-mesenchymal transition of human cardiac microvascular endothelial cells." International Journal of Molecular Medicine (2017). WB ; Human. PubMed:28534977 |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 肿瘤 细胞生物 免疫学 发育生物学 神经生物学 生长因子和激素 激酶和磷酸酶 细胞膜受体 细胞表面分子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Pig, Horse, |
| 产品应用 | ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 147kDa |
| 细胞定位 | 细胞浆 细胞膜 分泌型蛋白 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouae VEGFR1:1162-1260/1333 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: VEGF Receptor 1 (also known as FLT) belongs to the src gene family and shows tyrosine protein kinase activity that is important for the control of cell proliferation and differentiation. The protein acts as a receptor for VEGF, VEGFB and PGF. An alternatively spliced form of the gene produces a soluble protein (sFlt1) which binds vascular endothelial growth factor (VEGF) with high affinity. sFlt1 has a higher affinity for VEGF indicating that it may function as an inhibitor in the VEGF response. VEGF Receptor 1 is specifically expressed in most vascular endothelial cells and peripheral blood monocytes. Function: Tyrosine-protein kinase that acts as a cell-surface receptor for VEGFA, VEGFB and PGF, and plays an essential role in the development of embryonic vasculature, the regulation of angiogenesis, cell survival, cell migration, macrophage function, chemotaxis, and cancer cell invasion. May play an essential role as a negative regulator of embryonic angiogenesis by inhibiting excessive proliferation of endothelial cells. Can promote endothelial cell proliferation, survival and angiogenesis in adulthood. Its function in promoting cell proliferation seems to be cell-type specific. Promotes PGF-mediated proliferation of endothelial cells, and proliferation of some types of cancer cells, but does not promote proliferation of normal fibroblasts. Has very high affinity for VEGFA and relatively low protein kinase activity; may function as a negative regulator of VEGFA signaling by limiting the amount of free VEGFA and preventing its binding to KDR. Modulates KDR signaling by forming heterodimers with KDR. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate and the activation of protein kinase C. Mediates phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, leading to the activation of phosphatidylinositol kinase and the downstream signaling pathway. Mediates activation of MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Phosphorylates PLCG1. Promotes phosphorylation of AKT1, PTK2/FAK1; YES1 and CBL. Subunit: Interacts with VEGFA, VEGFB and PGF. Monomer in the absence of bound VEGFA, VEGFB or PGF. Homodimer in the presence of bound VEGFA, VEGFB and PGF. Can also form a heterodimer with KDR.Interacts (when tyrosine phosphorylated) with CBL, CRK, GRB2, NCK1,PIK3R1, PLCG1 and PTPN11. Interacts with GNB2L1/RACK1. Identified in a complex with CBL and CD2AP. Subcellular Location: Isoform 1: Cell membrane; Single-pass type I membrane protein. Endosome. Note=Autophosphorylation promotes ubiquitination and endocytosis；Isoform 2: Secreted；Isoform 3: Secreted；Isoform 4: Secreted；Isoform 5: Cytoplasm (Potential)；Isoform 6: Cytoplasm (Potential)；Isoform 7: Cytoplasm (Potential). Tissue Specificity: Detected in normal lung, but also in placenta,liver, kidney, heart and brain tissues. Specifically expressed in most of the vascular endothelial cells, and also expressed in peripheral blood monocytes. Isoform 2 is strongly expressed in placenta. Isoform 3 is expressed in corneal epithelial cells (at protein level). Isoform 3 is expressed in vascular smooth muscle cells (VSMC). Post-translational modifications: N-glycosylated. Ubiquitinated after VEGFA-mediated autophosphorylation, leading to proteolytic degradation. Autophosphorylated on tyrosine residues upon ligand binding. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit. Phosphorylation at Tyr-1169 is important for interaction with PLCG1. Phosphorylation at Tyr-1213 is important for interaction with PIK3R1, PTPN11, GRB2, and PLCG1. Phosphorylation at Tyr-1331 is important for endocytosis and for interaction with CBL, NCK1 and CRK. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.Contains 7 Ig-like C2-type (immunoglobulin-like)domains.Contains 1 protein kinase domain. SWISS: P53767 Gene ID: 14254 Database links: Entrez Gene: 2321 Human Entrez Gene: 14254 Mouse Entrez Gene: 54251 Rat Omim: 165070 Human SwissProt: P17948 Human SwissProt: P35969 Mouse SwissProt: P53767 Rat Unigene: 594454 Human Unigene: 389712 Mouse Unigene: 10239 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. VEGFR1/Flt1是一种细胞膜受体激酶，对血管内皮生长因子有高度的亲和性，主要功能是参与血管内皮细胞生长和血管生成的调控。用于各种恶性肿瘤的研究。 |
| 产品图片 |  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-VEGFR1/FLT1 Polyclonal Antibody, Unconjugated(bs-0170R) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control: mouse spleen cells(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green). |
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文献和实验相关实验
人血管内皮生长因子受体2(VEGFR2)酶联免疫分析（ELISA）
人血管内皮生长因子受体2(VEGFR2) 酶联免疫分析（ELISA ） 试剂盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中 血管内皮生长因子受体2(VEGFR2) 的 含量。 实验原理： 本试剂盒应用双抗体夹心法测定标本中人 血管内皮生长因子受体2(VEGFR2) 水平。用纯化的人 血管内皮生长因子受体2(VEGFR2) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中
小鼠 血管内皮生长因子受体2（VEGFR2) 酶联免疫 分析（ ELISA ） 试剂 盒使用说明书 本试剂仅供研究使用 目的：本试剂盒用于测定小鼠血清，血浆及相关液体样本中 血管内皮生长因子受体2（VEGFR2) 含量。 实验原理 ： 本试剂盒应用双抗体夹心法测定 标本 中 小鼠 血管内皮生长因子受体2（VEGFR2) 水平。用纯化的 小鼠 血管内皮生长因子受体2（VEGFR2) 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入
【求助】同一种受体，与不同的配体结合后是否可以产生相反的生物学活性？
huangdde 如血管内皮生长因子（VEGF）与其受体（VEGFR）结合后，会刺激细胞增殖，如下现象是否可以出现：某种物质与VEGFR结合后，该物质会通过VEGFR而抑制细胞增殖？ 谢谢。 holywater 一般来说受体结合是比较特异的，但是可能和结构类似的东西结合而促进或者抑制受体的活性，这也是竞争性抑制剂的来由。不过抑制剂不是“配体”，所以我说楼主的定义可能有些错误。
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VEGFR1血管内皮生长因子受体1抗体
¥780 - 2200
