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Selenoprotein W硒蛋白W抗体

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  • ¥1380 - 2200
  • LMAI Bio
  • LM-0495R
  • 进口/国产
  • 2025年07月11日
  • ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) 
  • Rabbit
  • Human, Mouse, Rat, Sheep, 
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 供应商

      上海联迈生物工程有限公司

    • 库存

      大量

    • 目录编号

      LM-0495R

    • 克隆性

      多克隆

    • 抗原来源

      Rabbit

    • 保质期

      1年

    • 抗体英文名

      Selenoprotein W

    • 抗体名

      硒蛋白W抗体

    • 宿主

      Rabbit

    • 适应物种

      Human, Mouse, Rat, Sheep, 

    • 免疫原

      KLH conjugated synthetic peptide derived from mouse Selenoprotein W:13-88/88 

    • 亚型

      IgG

    • 形态

      Lyophilized or Liquid

    • 应用范围

      ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) 

    • 浓度

      1mg/ml

    • 保存条件

      Store at -20 °C

    • 规格

      100ul  200ul

    Selenoprotein W硒蛋白W抗体
    英文名称 Selenoprotein W
    中文名称 硒蛋白W抗体
    别    名 MGC105482; SelW; SEPW1; Selenoprotein W; SELW_RAT; SELW_HUMAN.  
    规格价格 100ul/1380元 购买    200ul/2200元 购买    大包装/询价
    说 明 书 100ul  200ul
    研究领域  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat, Sheep, 
    产品应用 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 (石蜡切片需做抗原修复) 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 9.5kDa
    细胞定位 细胞浆 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from mouse Selenoprotein W:13-88/88 
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    This gene encodes a selenoprotein containing a selenocysteine (Sec) residue, which is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is highly expressed in skeletal muscle and brain. It belongs to the SelWTH family, which possesses a thioredoxin-like fold and a conserved CxxU (C is cysteine, U is Sec) motif, suggesting a redox function for this gene. Studies in mouse show that this selenoprotein is involved in muscle growth and differentiation, and in the protection of neurons from oxidative stress during neuronal development. A pseudogene for this locus has been identified on chromosome 10. [provided by RefSeq, May 2017].

    Function:
    Plays a role as a glutathione (GSH)-dependent antioxidant. May be involved in a redox-related process. May play a role in the myopathies of selenium deficiency.

    Subunit:
    Interacts with DPYSL2, PRDX1, YWHAB, YWHAG, HSP70 and HSP90.

    Subcellular Location:
    Cytoplasm.

    Tissue Specificity:
    Ubiquitously expressed with highest levels in skeletal muscle and heart, moderate levels in brain, spinal cord, thyroid, spleen, prostate, ovary, small intestine and colon, and lowest levels in liver and lymph node.

    Similarity:
    Belongs to the SelWTH family. SELW subfamily.

    SWISS:
    P63300

    Gene ID:
    20364

    Database links:

     



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

    硒蛋白-W(selenoprotein W)和一种存在于多种组织中的18ku硒蛋白P(18ku selenoprotein)。它们具有防止膜结构及生物大分子的氧化损伤;合成并调节DNA表达;促进精子生成、发育及维持其活力;保护内皮细胞和维护肌肉组织的正常功能等广泛的生理作用。该蛋白与人、羊有部分交叉。
    产品图片 产品细节图片1
    Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Selenoprotein W) Polyclonal Antibody, Unconjugated (bs-0495R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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    图标文献和实验
    相关实验
    • WW-value

      WW-value 由于放射线的作用,在物质中每生成一个离子对所消耗的射线的平均能量。它是一个与放射线的气体分解初期过程密切相关的数值。格雷(L.H.Gray.1936)首先测定了空气,后来又测定了很多其他气体,表明W值与射线的种类和能量(极端低的情况除外)关系不大,都是30eV左右(空气33.7,水蒸汽30.4)。由于离子化能(I)是15eV左右,过剩的能量(W-I)认为是消耗于2个左右的激发上。对固体和液体的测定非常困难。可认为W值是30eV或30eV以下(对水从G值可知W

    • BLOTTING W/ BSA

      Blotting with BSA Blot in 5% mile/ 1X PBST (or 1X TBST) --60'/Rt Wash 2X for 5' in 1XPBST 10Ab in 1% BSA/1X PBST --60'/Rt Wash PBST 15', then 3x for 5' each in 1X PBST 20Ab in 1% BSA/1X PBST --60'/Rt

    • W检验界值表

      佚名 n 3 4 5 6 7 8 9 10 11 12 13 14 α=10.0

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    Selenoprotein W硒蛋白W抗体
    ¥1380 - 2200