GAD65谷氨酸脱羧酶-65抗体

价格¥780 - 2200

品牌LMAI Bio
货号LM-0325R
产地进口/国产
更新日期2025年07月12日

应用范围WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
宿主Rabbit
适应物种Human, Mouse, Rat, Chicken, Pig,

上海联迈生物工程有限公司

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详细信息
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供应商：
上海联迈生物工程有限公司
库存：
大量
目录编号：
LM-0325R
克隆性：
多克隆
抗原来源：
Rabbit
保质期：
1年
抗体英文名：
GAD65
抗体名：
谷氨酸脱羧酶-65抗体
宿主：
Rabbit
适应物种：
Human, Mouse, Rat, Chicken, Pig,
亚型：
IgG
形态：
Lyophilized or Liquid
应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复）
浓度：
1mg/ml
保存条件：
Store at -20 °C
规格：
50ul 100ul 200ul

GAD65谷氨酸脱羧酶-65抗体

英文名称	GAD65
中文名称	谷氨酸脱羧酶-65抗体
别名	GAD65; 65 kDa glutamic acid decarboxylase; DCE 2; DCE2; GAD 2; GAD 65; GAD-2; GAD-65; GAD2; Glutamate Decarboxylase 2 (pancreatic islets and brain 65kDa); Glutamate Decarboxylase 2; Glutamate Decarboxylase 65; Glutamate decarboxylase 65 kDa isoform; Glutamic Acid Decarboxylase 2; Glutamic Acid Decarboxylase 65.
规格价格	50ul/780元购买 100ul/1380元购买 200ul/2200元购买大包装/询价
说明书	50ul 100ul 200ul
研究领域	免疫学糖尿病
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Chicken, Pig,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	65kDa
细胞定位	细胞浆细胞膜
性状	Lyophilized or Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human GAD65:501-585/585
亚型	IgG
纯化方法	affinity purified by Protein A
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Oct 2008] Function: Catalyzes the production of GABA. Subunit: Homodimer. Subcellular Location: Cytoplasm, cytosol. Cytoplasmic vesicle. Cell junction, synapse, presynaptic cell membrane; Lipid-anchor. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note=Associated to cytoplasmic vesicles. In neurons, cytosolic leaflet of Golgi membranes and presynaptic clusters. Post-translational modifications: Phosphorylated; which does not affect kinetic parameters or subcellular location. Palmitoylated; which is required for presynaptic clustering. Similarity: Belongs to the group II decarboxylase family. SWISS: Q05329 Gene ID: 2572 Database links: Entrez Gene: 2572 Human Entrez Gene: 14417 Mouse Entrez Gene: 24380 Rat Omim: 138275 Human SwissProt: Q05329 Human SwissProt: Q5VZ29 Human SwissProt: Q5VZ30 Human SwissProt: P48320 Mouse SwissProt: Q548L4 Mouse SwissProt: Q05683 Rat Unigene: 231829 Human Unigene: 4784 Mouse Unigene: 29951 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 谷氨酸脱羧酶-65(GAD65)是用于I II型糖尿病研究的很重要的蛋白。
产品图片	Sample: Hela Cell Lysate at 40 ug Primary: Anti- GAD65 (bs-0325R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size: 65 kD Observed band size: 65 kD Sample: Lung(Rat) lysate at 30 ug; Primary: Anti-GAD65 (bs-0325R) at 1:300 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000 dilution; Predicted band size : 65kD Observed band size : 65kD Sample: Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-GAD65 (bs-0325R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65 kD Sample: brain (Mouse) Lysate at 40 ug Primary: Anti-GAD65 (bs-0325R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 65 kD Observed band size: 65 kD Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-GAD65 Polyclonal Antibody, Unconjugated(bs-0325R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GAD65) Polyclonal Antibody, Unconjugated (bs-0325R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.