- ¥2980
- LMAI Bio
- LM-3134R-FITC
- 中国/美国/欧洲
- 2025年07月15日
- IF=1:50-200
- Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig,
- Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海联迈生物工程有限公司
- 库存：
大量
- 靶点：
详见说明书
- 级别：
1
- 目录编号：
LM-3134R-FITC
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
1年
- 抗体英文名：
Anti-Phospho-Ezrin (Thr567)/FITC
- 抗体名：
Anti-Phospho-Ezrin (Thr567)/FITC
- 标记物：
FITC标记
- 宿主：
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig,
- 适应物种：
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig,
- 免疫原：
详见说明书
- 亚型：
IGg
- 形态：
粉末、液体、冻干粉
- 应用范围：
IF=1:50-200
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
100ul
| 英文名称 | Anti-Phospho-Ezrin (Thr567)/FITC |
| 中文名称 | FITC标记的磷酸化埃兹蛋白抗体 |
| 别 名 | Ezrin (phospho T567); Ezrin (phospho Thr567); p-Ezrin (phospho T567); CVIL; CVL; cytovillin 2 ; Cytovillin ; DKFZp762H157; FLJ26216; MGC1584; p81; VIL 2 ; VIL2; Villin 2; Villin2; EZRI_HUMAN. |
| 规格价格 | 100ul/2980元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig, |
| 产品应用 | IF=1:50-200 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 64kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human Ezrin around the phosphorylation site of Thr567 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| 产品介绍 | background: The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. Function: Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis. Subunit: Interacts with MPP5 and SLC9A3R2. Found in a complex with EZR, PODXL and SLC9A3R2 (By similarity). Interacts with MCC, PLEKHG6, PODXL, SCYL3/PACE1, SLC9A3R1 and TMEM8B. Interacts (when phosphorylated) with FES/FPS. Subcellular Location: Apical cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection. Cell projection, microvillus membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, ruffle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Note=Localization to the apical membrane of parietal cells depends on the interaction with MPP5. Localizes to cell extensions and peripheral processes of astrocytes. Microvillar peripheral membrane protein (cytoplasmic side). Tissue Specificity: Expressed in cerebral cortex, basal ganglia, hippocampus, hypophysis, and optic nerve. Weakly expressed in brain stem and diencephalon. Stronger expression was detected in gray matter of frontal lobe compared to white matter (at protein level). Component of the microvilli of intestinal epithelial cells. Preferentially expressed in astrocytes of hippocampus, frontal cortex, thalamus, parahippocampal cortex, amygdala, insula, and corpus callosum. Not detected in neurons in most tissues studied. Post-translational modifications: Phosphorylated by tyrosine-protein kinases. Phosphorylation by ROCK2 suppresses the head-to-tail association of the N-terminal and C-terminal halves resulting in an opened conformation which is capable of actin and membrane-bindin. Similarity: Contains 1 FERM domain. Database links: Entrez Gene: 7430 Human Entrez Gene: 281574 Cow Entrez Gene: 22350 Mouse Entrez Gene: 54319 Rat Omim: 123900 Human SwissProt: P31976 Cow SwissProt: P15311 Human SwissProt: P26040 Mouse SwissProt: P31977 Rat Unigene: 487027 Human Unigene: 277812 Mouse Unigene: 773 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 信号传导（Signaling Intermediates） 目前研究发现Ezrin蛋白与维持细胞的形状、极性、生长运动以及信号转导方面发挥重要作用，该蛋白与肿瘤的侵袭、转移有关。 |
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文献和实验sunchangzheng 我打算用此抗体通过流式检测细胞表面P糖蛋白的表达量差异。 说明书中说是多抗，这种抗体有单抗的吧？ 用于FCM： 1：20-100，这是说用于流式的稀释倍数，是不是稀释倍数越小越好呢？ 同型对照抗体是不是必须要做的？如何选择阴型同型对照抗体呢！ 我用的抗体是FITC标记的P-糖蛋白抗体 谢谢交流与帮助！ freecell 抗体的稀释倍数是需要优化
的研究已经成为蛋白质科学的热点之一。 磷酸化多肽（主要指肽链中的酪氨酸、丝氨酸和苏氨酸残基的侧链羟基被磷酸化生成酸式磷酸酯的修饰多肽）是研究蛋白质磷酸化过程的必不可少的工具，它可作为磷酸酶模型底物，或作为可产生抗磷酸化蛋白抗体的抗原，也可以在确定磷酸化蛋白的物理参数时作为参考化合物等[3]。因此磷酸化多肽的合成在过去的几年中吸引了相当大的兴趣，目前已确定了较为成熟的合成路线，使磷酸化多肽的合成趋于常规。目前磷酸化多肽的合成主要有两个策略：后磷酸化法（Global
抗体—抗体复合物，再用水洗去未反应的标记抗体，干燥、封片后镜检。如果检查未知抗体，则表明抗原标本是已知的，待检血清为第一抗体，其它步骤的抗原检查相同。标记的抗抗体是抗球蛋白抗体，同于血清球蛋白有种的特异性，如免疫抗鸡血清球蛋白只对鸡的球蛋白发生反应，因此，制备标记抗体适用于鸡任何抗原的诊断。2．技术分类： ⑴ 荧光抗体技术（荧光显微镜技术） 抗原抗体反应后，利用荧光显微镜判定结果的检测方法。 ⑵ 免疫荧光测定技术： 抗原抗体反应后，利用特殊仪器测定荧光强度而推算被测物浓度的检测
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