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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
Anti-Histone H4抗体- ChIP Grade
- 宿主:
Rabbit
- 库存:
需确认
- 保质期:
见产品外包装
- 供应商:
嵘崴达
- 是否单克隆:
多克隆
- 保存条件:
见产品外包装
- 亚型:
IgG
- 规格:
100 µg
Anti-Histone H4抗体- ChIP Grade (ab10158)
-
产品名称
Anti-Histone H4抗体- ChIP Grade
描述 -
兔多克隆抗体to Histone H4 - ChIP Grade
-
宿主
Rabbit -
特异性
From May 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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经测试应用
适用于: IP, ChIP, IHC-P, WB
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文献和实验Chromatin Immunoprecipitation (ChIP)
of formaldehyde in the ChIP method was pioneered by Solomon and Varshavsky (Solomon et al. 1988). In their original studies, the association of histone H4 and RNA polymerase II with the Drosophila hsp70 genes was monitored. Cells were treated with formaldehyde
条件有待改进) 2、阳性对照:一般用anti-RNA Polymerase II抗体,因为RNA Polymerase II是通用转录因子, 在所有细胞中都能结合基因(当然是在该细胞中表达的基因,所以为了保证阳性对照有效,一般选择 阳性对照的引物是根据管家基因设计的)的核心启动子区,因此,理论上ChIP后PCR都会有条带 3、阴性对照:用普通的IgG做为抗体,理论上不会ChIP下来任何DNA片段,因此作为阳性对照, 但是由于非特异结合,或者实验过程中,没发生结合的DNA清除不完
Whole genome amplification protocol for ChIP-chip
of different factors (E2F family members, KAP1, CtBP2, ZNF217) as well as on histone modifications (H3me3K9, H3me3K27, H3me3K4) (Krig et al., 2007; O'Geen et al., 2007). Another benefit of the WGA amplification method is the ability to perform a second
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