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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
CD19 Monoclonal Antibody (eBio1D3 (1D3)), PE, eBioscience™
- 浓度:
0.2 mg/mL
- 宿主:
Rat
- 供应商:
嵘崴达
- 保质期:
见产品说明书
- 适应物种:
见产品说明书
- 级别:
见产品说明书
- 库存:
需确认
- 标记物:
见产品说明书
- 是否单克隆:
单克隆
- 保存条件:
见产品说明书
- 亚型:
IgG2a
- 规格:
500 ug
CD19 Monoclonal Antibody (eBio1D3 (1D3)), PE, eBioscience™
货号 12-0193-85产品描述
Description: The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.
Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.
Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Intracellular Immunofluorescent Staining for Flow Cytometry
Right: Mouse splenocytes were stimulated for 3 days with IL-2. Cells were surface stained with APC CD49d (DX5) (Cat. No. 17-5971 ) and subsequently stained intracellularly with PE GB11. Human IL-17 (eBio64CAP17): Human
Fluorochrome Absorption and Emission Spectra
conjugates to monoclonal antibodies and other proteins. Four of these fluorochromes, fluorescein isothiocyanate (FITC), R-phycoerythrin (R-PE), BD Cy-Chrome™, and PerCP, can be used with single-laser flow cytometers equipped with an argon-ion laser emitting
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