IL-2 Human Uncoated ELISA Kit

IL-2 Human Uncoated ELISA Kit with Plates

货号 88-7025-22

The Human Interleukin-2 (IL-2) Uncoated ELISA Kit contains pre-matched antibody pairs, plates and reagents for performing quantitative enzyme linked immunosorbent assays (ELISA) to detect and quantify protein levels of Humanmouse IL-2. Wash Buffer and Stop Solution are needed to complete the ELISA reaction and are sold separately.

原理方法

ELISAs are designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody is coated to the bottom of the wells of a microplate, which is an overnight process. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. A sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.

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