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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名:
CD152 (CTLA-4) Monoclonal Antibody (UC10-4B9), Biotin, eBioscience™
- 浓度:
0.5 mg/mL
- 宿主:
Armenian hamster
- 供应商:
嵘崴达
- 适应物种:
见产品说明书
- 库存:
需确认
- 保质期:
见产品说明书
- 抗原来源:
见产品说明书
- 标记物:
见产品说明书
- 是否单克隆:
单克隆
- 保存条件:
4° C
- 规格:
100 ug
货号 13-1522-82
产品描述
Description: The UC10-4B9 monoclonal antibody reacts with mouse CD152, also known as the cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells at low level and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cells to an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in a large proportion of newly synthesized CTLA-4 in response to TCR signaling to be localized intracellularly.
Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.
Applications Reported: The UC10-4B9 antibody has been reported for use in flow cytometric analysis.
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文献和实验细胞的状态,是否形成克隆团,或进行细胞计数,如果出现培养基变黄或细胞密度过高的情况,吹散细胞克隆团后调整细胞密度至0.5-1×10^6/ml,更换培养板和培养基; 4. 移除 CD3/CD28 Streptamers 终止刺激(该步根据实验需求可选做):直接向刺激后的细胞悬液中加入终浓度为 1mM 的 Biotin,室温孵育 30 分钟。收集并转移细胞至 15ml 离心管中,加入 10ml 培养基,300g 离心 6-10 分钟,弃上清,再次加入 10ml 培养基重复一遍洗涤步骤,至此,细胞已经可以用于
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
/Western blotting assays. Another factor important in achieving optimal results is the degree of sensitivity of cells to the antibody-induced apoptosis. As an example, CD95 (Fas)-induced apoptosis of human Jurkat cell line by monoclonal antibody EOS
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