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- eBioscience
- 12-0251-81
- 原装进口
- 2025年07月16日
- Rat
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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名：
CD25 Monoclonal Antibody (PC61.5), PE, eBioscience™
- 浓度：
0.2 mg/mL
- 宿主：
Rat
- 供应商：
嵘崴达
- 库存：
需确认
- 适应物种：
见产品说明书
- 保质期：
见产品说明书
- 抗原来源：
见产品说明书
- 标记物：
见产品说明书
- 是否单克隆：
单克隆
- 保存条件：
4° C
- 亚型：
IgG1
- 亚型：
IgG1
- 规格：
50 ug
CD25 Monoclonal Antibody (PC61.5), PE, eBioscience™
货号 12-0251-81产品描述
Description: The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2R alpha). CD25 is expressed by early progenitors of the T and B lineage as well as by activated mature T and B lymphocytes. By itself, CD25 binds IL-2 only with low affinity. However, CD25 associates with CD122 (IL-2 receptor beta chain) and CD132 (common gamma chain) to form the high affinity IL-2 receptor. Binding of IL-2 to both the high and low affinity classes of IL-2 receptor is inhibited by the PC61.5 antibody. CD25 plays a role in lymphocyte differentiation and activation/proliferation.
Applications Reported: The PC61.5 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The PC61.5 antibody has been tested by flow cytometric analysis of mouse splenocytes and Con A-stimulated splenocyte culture. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
Intracellular Cytokine Staining Protocol
anti-cytokine antibodies with excess amounts of unlabeled antibody. Alternatively, recombinant cytokines can be used for blocking.eBioscience antibodies are available as Functional Grade (FG) Purified (sterile, endotoxin-tested, no azide), Purified
Fluorochrome Absorption and Emission Spectra
conjugates to monoclonal antibodies and other proteins. Four of these fluorochromes, fluorescein isothiocyanate (FITC), R-phycoerythrin (R-PE), BD Cy-Chrome™, and PerCP, can be used with single-laser flow cytometers equipped with an argon-ion laser emitting
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