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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
IL-2 Monoclonal Antibody (MQ1-17H12), PE, eBioscience™
- 浓度:
5 µL/Test
- 宿主:
Rat
- 库存:
需确认
- 供应商:
嵘崴达
- 保质期:
见产品外包装
- 是否单克隆:
单克隆
- 保存条件:
4° C
- 亚型:
IgG2a
- 规格:
100 tests
IL-2 Monoclonal Antibody (MQ1-17H12), PE, eBioscience™
货号 12-7029-42
产品详细信息
Description: The MQ1-17H12 antibody reacts with human interleukin-2 (IL-2), a 17 kDa T cell growth factor and a major immunoregulatory cytokine. The MQ1-17H12 antibody is a non-neutralizing antibody.
Applications Reported: MQ1-17H12 has been reported for use in intracellular flow cytometric analysis.
Applications Tested: This MQ1-17H12 antibody is offered in 2 formats: - µg size: has been tested by intracellular staining of stimulated normal human perioheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining of stimulated normal human perioheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
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文献和实验Intracellular Cytokine Staining Protocol
IL-2 PBMC PMA (30-50ng/ml)/Iono (1ug/ml) 5hr - Monensin MQ1-17H12 IL-4 PBMC anti-CD3 (10µg/ml, immobilized) + anti
Intracellular Immunofluorescent Staining for Flow Cytometry
MQ1-17H12 IL-4 PBMC anti-CD3 (10µg/ml, immobilized) + anti-CD28 (2µg/ml, soluble) + IL-2 (10ng/ml) + IL-4 (20ng/ml) (2d); IL-2 (10ng/ml) + IL-4 (20ng/ml) (3d) 2d/3d PMA (5ng/ml) + Ionomycin
secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
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