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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海康朗生物科技有限公司
- 库存:
大量
- 目录编号:
kl-17422R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
12个月
- 抗体英文名:
Histone H3.1 antibody
- 抗体名:
组蛋白H3.1抗体
- 宿主:
Rabbit
- 适应物种:
Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep, Chimpanzee,
- 免疫原:
KLH conjugated synthetic peptide derived from human Histone H3.1:71-136/136
- 亚型:
IgG
- 形态:
冻干粉或液体
- 应用范围:
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500
- 浓度:
1mg/ml
- 保存条件:
-20 °C
- 规格:
100ul 200ul
| 中文名称 | 组蛋白H3.1抗体 |
| 别 名 | H3 histone family, member A; H3 histone family, member B; H3 histone family, member C; H3 histone family, member D; H3 histone family, member E; H3 histone family, member F; H3 histone family, member G; H3 histone family, member H; H3 histone family, member I; H3 histone family, member J; H3 histone family, member K; H3 histone family, member L; H3.1; H3.f; H3/A; H3/c; H3/d; H3/f; H3/h; H3/i; H3/j; H3/k; H3/l; H31_HUMAN; H3F1K; H3FA; H3FB; H3FC; H3FD; H3FF; H3FH; H3FI; H3FJ; H3FK; H3FL; HIST1 cluster H3A; HIST1 cluster H3B; HIST1 cluster H3C; HIST1 cluster H3D; HIST1 cluster H3E; HIST1 cluster H3F; HIST1 cluster H3G; HIST1 cluster H3I; HIST1 cluster H3J; HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; histone 1, H3a; histone 1, H3b; Histone 1, H3c; Histone 1, H3e; histone 1, H3f; histone 1, H3g; histone 1, H3h; histone 1, H3i; histone 1, H3j; histone cluster 1, H3a; histone cluster 1, H3b; Histone cluster 1, H3c; Histone cluster 1, H3e; histone cluster 1, H3g; histone cluster 1, H3h; histone cluster 1, H3i; histone cluster 1, H3j; Histone gene cluster 1 H3A; Histone gene cluster 1 H3B; Histone gene cluster 1 H3E; Histone gene cluster 1 H3F; Histone gene cluster 1 H3G; Histone gene cluster 1, H3; Histone gene cluster 1, H3C; Histone gene cluster 1, H3D; Histone gene cluster 1, H3I; Histone gene cluster 1, H3IH; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l |
| 规格价格 | 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 100ul 200ul |
| 研究领域 | 细胞生物 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Pig, Cow, Horse, Rabbit, Sheep, Chimpanzee, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 15kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Histone H3.1:71-136/136 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq, Jul 2008] Function: Histone H3.1 is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodelling. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3 H4 heterotetramer and two H2A H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Subcellular Location: Nuclear Similarity: Belongs to the histone H3 family. Gene ID: 8350 Database links: Entrez Gene: 8350 Human Entrez Gene: 8351 Human Entrez Gene: 8352 Human Entrez Gene: 8353 Human Entrez Gene: 8354 Human Entrez Gene: 8355 Human Entrez Gene: 8356 Human Entrez Gene: 8357 Human Entrez Gene: 8358 Human Entrez Gene: 8968 Human Entrez Gene: 319148 Mouse Entrez Gene: 319151 Mouse Entrez Gene: 319152 Mouse Entrez Gene: 319153 Mouse Entrez Gene: 360198 Mouse Entrez Gene: 97908 Mouse Omim: 602810 Human Omim: 602811 Human Omim: 602812 Human Omim: 602813 Human Omim: 602814 Human Omim: 602815 Human Omim: 602816 Human Omim: 602817 Human Omim: 602818 Human Omim: 602819 Human SwissProt: P68431 Human SwissProt: P68433 Mouse Unigene: 132854 Human Unigene: 247813 Human Unigene: 247814 Human Unigene: 248176 Human Unigene: 443021 Human Unigene: 484990 Human Unigene: 532144 Human Unigene: 533292 Human Unigene: 546315 Human Unigene: 586261 Human Unigene: 591778 Human Unigene: 626666 Human Unigene: 221301 Mouse Unigene: 261657 Mouse Unigene: 377874 Mouse Unigene: 390558 Mouse Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品图片 | ![]() Sample:A549 Cell (Human) Lysate at 40 ug Primary: Anti-Histone H3.1(bs-17422R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15kD Observed band size: 15kD ![]() Sample:TT Cell (Human) Lysate at 40 ug Primary: Anti-Histone H3.1(bs-17422R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15kD Observed band size: 15kD ![]() Sample:Stomach (Mouse) Lysate at 40 ug Primary: Anti-Histone H3.1(bs-17422R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15kD Observed band size: 15kD ![]() Sample:U251 Cell (Human) Lysate at 40 ug Primary: Anti-Histone H3.1(bs-17422R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15kD Observed band size: 15kD ![]() Sample:A431 Cell(Human)Lysate at 40 ug Primary: Anti-Histone H3.1(bs-17422R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15kD Observed band size: 15kD |
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文献和实验Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
. The protocol described here allows the detection of phosphorylated histones in tissue-cultured cells and tissue sections by fluorescent or bright-field immunostaining analysis. Here we used a serine 10 specific P-histone H3 antibody to determine
FACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic Cells
scanner (FACS) is described, based on the presence of an intranuclear antigen present only in mitotic cells, detected using a specific, commercially available antibody. Cell staining and FACS analysis can be done in a single day, making this a rapid
Genome-Wide Measurement of Histone H3 Replacement Dynamics in Yeast
chromatin. Understanding the dynamic behavior of chromatin is of great interest for fields ranging from transcriptional regulation through meiosis and gametogenesis. Here, we describe a protocol for measuring histone replacement rates genome wide
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