E2F5 antibody

价格¥780 - 2200

品牌康朗生物
货号kl-1734R
产地中国/美国/德国
更新日期2026年01月07日

应用范围WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500
宿主Rabbit
适应物种Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse,

上海康朗生物科技有限公司

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供应商：
上海康朗生物科技有限公司
库存：
大量
目录编号：
kl-1734R
克隆性：
多克隆
抗原来源：
Rabbit
保质期：
12个月
抗体英文名：
E2F5 antibody
抗体名：
转录因子E2F-5抗体
宿主：
Rabbit
适应物种：
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse,
免疫原：
KLH conjugated synthetic peptide derived from human E2F5:101-200/346
亚型：
IgG
形态：
冻干粉或液体
应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500
浓度：
1mg/ml
保存条件：
-20 °C
规格：
50ul 100ul 200ul

E2F5 antibody

中文名称	转录因子E2F-5抗体
别名	E2F-5; transcription factor E2F5; E2F 5; E2F transcription factor 5; E2F transcription factor 5 p130 binding; p130 binding; E2F5_HUMAN.
	Specific References (1) \| bs-1734R has been referenced in 1 publications. [IF=2.15] Zhao, Jin, et al. "Analysis of genetic aberrations on chromosomal region 8q21?C24 identifies E2F5 as an oncogene with copy number gain in prostate cancer."Medical Oncology 30.1 (2013): 1-10.. WB ; Human. PubMed:23377984
规格价格	50ul/780元购买 100ul/1380元购买 200ul/2200元购买大包装/询价
说明书	50ul 100ul 200ul
研究领域	免疫学转录调节因子
抗体来源	Rabbit
克隆类型	Polyclonal
交叉反应	Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse,
产品应用	WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
分子量	37kDa
性状	Lyophilized or Liquid
浓度	1mg/ml
免疫原	KLH conjugated synthetic peptide derived from human E2F5:101-200/346
亚型	IgG
纯化方法	affinity purified by Protein A
储存液	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件	Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed	PubMed
产品介绍	background: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionarily conserved domains that are present in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein is differentially phosphorylated and is expressed in a wide variety of human tissues. It has higher identity to E2F4 than to other family members. Both this protein and E2F4 interact with tumor suppressor proteins p130 and p107, but not with pRB. Alternative splicing results in multiple variants encoding different isoforms. Function: Transcriptional activator that binds to E2F sites, these sites are present in the promoter of many genes whose products are involved in cell proliferation. May mediate growth factor-initiated signal transduction. It is likely involved in the early responses of resting cells to growth factor stimulation. Subunit: Component of the DRTF1/E2F transcription factor complex. Binds cooperatively with DP-1 to E2F sites. Interaction with retinoblastoma protein RB1 or proteins RBL1 and RBL2 inhibits the E2F transactivation domain. Component of the DREAM complex (also named LINC complex) at least composed of E2F4, E2F5, LIN9, LIN37, LIN52, LIN54, MYBL1, MYBL2, RBL1, RBL2, RBBP4, TFDP1 and TFDP2. The complex exists in quiescent cells where it represses cell cycle-dependent genes. It dissociates in S phase when LIN9, LIN37, LIN52 and LIN54 form a subcomplex that binds to MYBL2. Subcellular Location: Nucleus. Similarity: Belongs to the E2F/DP family. SWISS: Q15329 Gene ID: 1875 Database links: Entrez Gene: 1875 Human Omim: 600967 Human SwissProt: Q15329 Human Unigene: 445758 Human Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. E2F5转录因子具有调控细胞增殖、分化和凋亡相关基因转录的重要作用.
产品图片	Sample: RSC96(Rat) Cell Lysate at 30 ug Primary: Anti-E2F5 (bs-1734R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 37 kD Observed band size: 34 kD Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (E2F5) Polyclonal Antibody, Unconjugated (bs-1734R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Blank control: RSC96(blue), the cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice. Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 1μg in 100 μL1X PBS containing 0.5% BSA(green).