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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
上海康朗生物科技有限公司
- 库存:
大量
- 目录编号:
kl-9450R
- 克隆性:
多克隆
- 抗原来源:
Rabbit
- 保质期:
12个月
- 抗体英文名:
5 MethylCytosine antibody
- 抗体名:
5甲基胞嘧啶抗体
- 宿主:
Rabbit
- 适应物种:
Human
- 免疫原:
KLH conjugated 5 MethylCytosine:
- 亚型:
IgG
- 形态:
冻干粉或液体
- 应用范围:
ELISA=1:500-1000
- 浓度:
1mg/ml
- 保存条件:
-20 °C
- 规格:
100ul
| 中文名称 | 5甲基胞嘧啶抗体 |
| 别 名 | 5-Methyl Cytidine; 5 m C; 5 mC; 5 me C; 5 Me Cytidine; 5 MeCyd; 5 Methyl Cytidine; 5 MethylCytosine; 5-mC; 5-Me Cytidine; 5-Methyl-Cytosine; 5-MethylCytidine. |
| 规格价格 | 100ul/1580元 购买 大包装/询价 |
| 说 明 书 | 100ul |
| 产品类型 | 药物与化合物抗体 |
| 研究领域 | 细胞生物 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | 5 MethylCytosine |
| 产品应用 | ELISA=1:500-1000 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 0.16159kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated 5 MethylCytosine: |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 癈 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20癈. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 癈. |
| PubMed | PubMed |
| 产品介绍 | background: Cytidine is a nucleoside formed by a cytosine attached to a ribose ring via a beta-N1-glycosidic bond. DNA is methylated on cytidines by DNA methylases (DNMTs)to generate 5-methylcytidine (5-mC), a potent epigenetics marker and regulator of gene expression. The reverse reaction (cytidine demethylation) starts with its oxidation to hydroxymethyl- (5-hmC), formyl- (5-fC), and carboxy- (5-caC) cytidine. Several enzymes, including the Tet family of proteins have been implicated in cytidine demethylation. Subcellular Location: Nuclear. SWISS: N/A CAS: 58366-64-6 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
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文献和实验-methylcytosine antibody to immunoprecipitate DNA that contains highly methylated CpG sites. Enriched methylated DNA can be interrogated using DNA microarrays or by massive parallel sequencing techniques. This combined approach allows researchers to rapidly
is mrr+, so methylated adenines will be restricted. Clone can carry methylation activity.) (10) 3. In general, it is best to use a strain lacking Mcr and Mrr systems when cloning genomic DNA from an organism with methylcytosine such as mammals
and Mrr systems when cloning genomic DNA from an organism with methylcytosine such as mammals, higher plants , and many prokaryotes.(11) 4. The use of D(mrr-hsd-mcrB) hosts=general methylation tolerance and suitability for clones with N6
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