abcam/ Monensin Solution (1,0

Monensin Solution (1,000X) (ab193381)

• 产品名称

  Monensin Solution (1,000X)

• 产品概述

  Formulation: Monensin Solution is supplied as a 1000X working solution in 70% Ethanol. Dilute to 1X in cell culture medium.
  Concentration: 2.0 mM
  Storage & Handling: Store the solution between 2°C and 8°C. Monensin is known to be toxic; avoid direct body contact. Ethanol is flammable; keep away from sources of fire.
  Application: ICFC - Quality tested

  Recommended Usage: Dilute the 1000X solution to 1X in the tissue culture medium. It is recommended that cells are cultured with monensin for ≤ 24 hours, as this can become toxic for cell viability.

  • 说明

    Monensin is a protein transport inhibitor that increases intracellular cytokine staining signals by blocking transport processes during activation of a cell. This leads to the accumulation of most cytokines at the Golgi Complex/Endoplasmic Reticulum, and makes Monensin useful in intracellular staining of cytokines. Optimal conditions for use are cell type and time-dependent. Typically, protein transport inhibitors are included during in vitro cell activation cultures for 4-24 hours prior to harvest. Monensin Solution is supplied as a 1,000X solution, which should be diluted to 1X in cell culture medium.

     

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