- ¥780 - 2200
- 康朗生物
- kl-1573R
- 中国/美国/德国
- 2025年07月13日
- WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500
- Rabbit
- Human, Mouse, Rat, Dog, Pig, Cow,
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海康朗生物科技有限公司
- 库存：
大量
- 目录编号：
kl-1573R
- 克隆性：
多克隆
- 抗原来源：
Rabbit
- 保质期：
12个月
- 抗体英文名：
BBC3/PUMA antibodyBBC3/PUMA antibody
- 抗体名：
p53正向细胞凋亡调控因子抗体
- 宿主：
Rabbit
- 适应物种：
Human, Mouse, Rat, Dog, Pig, Cow,
- 免疫原：
KLH conjugated synthetic peptide derived from human BBC3:131-180/193
- 亚型：
IgG
- 形态：
冻干粉或液体
- 应用范围：
WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500
- 浓度：
1mg/ml
- 保存条件：
-20 °C
- 规格：
50ul 100ul 200ul
| 中文名称 | p53正向细胞凋亡调控因子抗体 |
| 别 名 | PUMA; bcl-2 binding component 3; BBC 3; BBC3; BCL 2 binding component 3; BCL2 binding component 3; JFY 1; JFY1; p53 up regulated modulator of apoptosis; p53 Upregulated Modulator of Apoptosis; PUMA alpha; PUMA/JFY1; BBC3_RAT. |
 |
Specific References (2) | bs-1573R has been referenced in 2 publications. [IF=1.96] Zhang, Pei, et al. "Epithelial-mesenchymal transition is necessary for acquired resistance to cisplatin and increases the metastatic potential of nasopharyngeal carcinoma cells." International Journal of Molecular Medicine (2013). WB ; Human. PubMed:24173500 [IF=5.62] Qiu, Shi, et al. "A small peptide derived from p53 linker region can resume the apoptotic activity of p53 by sequestering iASPP with p53." Cancer Letters 356.2 (2015): 910-917. IP ; Human. PubMed:25444901 |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 肿瘤 细胞生物 免疫学 细胞凋亡 线粒体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, Dog, Pig, Cow, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 21kDa |
| 细胞定位 | 细胞浆 线粒体 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BBC3:131-180/193 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: This gene encodes a member of the BCL-2 family of proteins. This family member belongs to the BH3-only pro-apoptotic subclass. The protein cooperates with direct activator proteins to induce mitochondrial outer membrane permeabilization and apoptosis. It can bind to anti-apoptotic Bcl-2 family members to induce mitochondrial dysfunction and caspase activation. Because of its pro-apoptotic role, this gene is a potential drug target for cancer therapy and for tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011] Function: Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Essential for ER stress-induced cell death. Subunit: Interacts with BCL2, BCL2L1/BCL-XL, MCL1 and BCL2A1. Subcellular Location: Mitochondrion. Note=Localized to the mitochondria in order to induce cytochrome c release. Similarity: Belongs to the Bcl-2 family. SWISS: Q96PG8 Gene ID: 27113 Database links: Entrez Gene: 27113 Human Entrez Gene: 170770 Mouse Entrez Gene: 317673 Rat Omim: 605854 Human SwissProt: Q9BXH1 Human SwissProt: Q99ML1 Mouse SwissProt: Q80ZG6 Rat Unigene: 467020 Human Unigene: 7660 Mouse Unigene: 25176 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PUMA在由p53诱导而经历细胞凋亡的细胞中被p53活化。在线粒体，PUMA 诱导细胞色素C的释放和激活对细胞凋亡的快速诱导。PUMA 与Bcl2、Bcl-XL、类Bcl2-1蛋白相互作用，促使线粒体易位和BAX的多聚化。PUMA在通过依赖细胞色素C/APAF1 途径介导p53诱导的细胞死亡中也发挥作用。 |
| 产品图片 |  Sample: K562 Cell Lysate at 30 ug Primary: Anti- BBC3 (bs-1573R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 21 kD Observed band size: 25 kD  Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BBC3/PUMA Polyclonal Antibody, Unconjugated(bs-1573R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Blank control: U-87MG(blue). Primary Antibody:Rabbit Anti-BBC3 PUMA antibody(bs-1573R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (bs-1573R,1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed. |
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