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- 文献和实验
- 技术资料
- 库存:
需确认
- 供应商:
嵘崴达
- 英文名:
Mouse GM-CSF Recombinant Protein, PeproTech®
- 规格:
5ug
Product Details
Description:
GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages, and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. The human and murine molecules are species-specific and exhibit no cross-species reactivity. Recombinant Murine GM-CSF is a 14.2 kDa globular protein consisting of 125 amino acid residues.
Source: E.coli
Synonyms: Granulocyte-Macrophage Colony-Stimulating Factor, CSF-2, MGI-1GM, Pluripoietin-α
AA Sequence: MAPTRSPITV TRPWKHVEAI KEALNLLDDM PVTLNEEVEV VSNEFSFKKL TCVQTRLKIF EQGLRGNFTK LKGALNMTAS YYQTYCPPTP ETDCETQVTT YADFIDSLKT FLTDIPFECK KPVQK
Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.
Biological Activity: The ED50 as determined by the dose-dependent stimulation of the proliferation of murine FDC-P1 cells is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 2 x 107 units/mg.
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文献和实验Synthesis of Recombinant Human Cytokine GM-CSF in the Seeds of Transgenic Tobacco Plants
We are interested in studying plant systems as vehicles for the production of recombinant proteins of clinical relevance. There are a number of potential advantages to producing recombinant protein products in plants. Plants are more economical
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Protein(91108ES)和HiActi® Recombinant Mouse IL-4 Protein(90144ES)进行小鼠骨髓来源树突状细胞(BMDCs)的体外诱导培养。实验方法显示:将C57BL/6小鼠骨髓细胞接种于含20 ng/mL GM-CSF、10 ng/mL IL-4的RPMI 1640完全培养基中,于第6天收集悬浮及弱贴壁细胞,用于后续RL-LNPs与PEG-LNPs处理、树突状细胞成熟检测、细胞因子检测及蛋白质组学等实验
Dynabeads® Mouse T-Activator CD3/CD28 – for physiological activation of mouse T cells
RPMI Medium 1640 with 2 mM L-Glutamin, 10% FCS/FBS and 100 U/ml penicillin/streptomycin can be used, or an equivalent culture medium. Heat inactivated Fetal Calf Serum (FCS). Recombinant mouse IL-2 (human IL-2 can also be used).
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