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- 详细信息
- 文献和实验
- 技术资料
- 抗体英文名:
IL-10 Monoclonal Antibody (JES3-9D7), PE, eBioscience™
- 浓度:
0.2 mg/mL
- 应用范围:
见说明书
- 宿主:
Rat
- 供应商:
嵘崴达
- 库存:
需确认
- 保质期:
见产品说明书
- 标记物:
见产品说明书
- 是否单克隆:
单克隆
- 保存条件:
4° C
- 亚型:
IgG1
- 规格:
100ug
IL-10 Monoclonal Antibody (JES3-9D7), PE, eBioscience™
货号 12-7108-82
产品描述
Description: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10).
Applications Reported: JES3-9D7 has been reported for use in intracellular staining and flow cytometric analysis.
Applications Tested: This JES3-9D7 antibody is offered in 2 formats: - µg size: has been tested by intracellular staining and flow cytometric analysis of human activated cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. - test size: has been pre-titrated and tested by intracellular staining and flow cytometric analysis of human activated cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
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文献和实验T-cell dependent antigen specific in situ staining (Pe)
and then apply 3% human serum as a blocking agent (health hazard!). 4- incubate with the blocking for 10 min. 5- blot the slides without washing and apply Phycoerythrin (B-Pe or R-Pe) 0.5µg/ml in PBS-BSA-NaN 3 , in a moist chamber, at RT for 1-18 hr.
ELISPOT (Enzyme-linked ImmunoSPOT)
5 88-7859 TRFK5 JES1-5A10 IL-6 88-7869 MQ2-13A5 MQ2-39C3 IL-10 88-7805 JES3-9D7 JES3-12G8 IL-12 p70 88
Intracellular Cytokine Staining Protocol
- Monensin JES3-9D7 IL-12 PBMC hIFNg (100ng/ml) (2hr)/LPS (100ng/ml) (22hr) 2hr/22hr - Monensin C8
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