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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 抗体英文名：
CD19 Monoclonal Antibody (HIB19), FITC, eBioscience™
- 浓度：
5 µL/Test
- 宿主：
Mouse
- 供应商：
嵘崴达
- 保质期：
见产品说明书
- 抗原来源：
见产品说明书
- 库存：
需确认
- 适应物种：
见产品说明书
- 标记物：
见产品说明书
- 是否单克隆：
单克隆
- 保存条件：
4° C
- 亚型：
IgG1
- 规格：
100 tests
CD19 Monoclonal Antibody (HIB19), FITC, eBioscience™
货号 11-0199-42产品描述
Description: The HIB19 monoclonal antibody reacts with human CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, Leu13, MHC class II, and CD19 form a multimolecular complex that associates with BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells. The SJ25C1 antibody and the HIB19 monoclonal antibody recognize overlapping epitopes.
Applications Reported: The HIB19 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HIB19 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
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- 作者
- 内容
- 询问日期
文献和实验secondary antibody review -- data from 99 publications
Signaling 18 IgG Alexa Fluor 488 immunocytochemistry 1:2000 18 IgG Alexa Fluor 568 immunocytochemistry 1:2000 19 donkey IgG HRP western blot 1:3000 detect antibody binding in mouse epithelial cells Amersham Biosciences 19 goat
Intracellular Cytokine Staining Protocol
Lymphocytes CD19 HIB19 14-0199 Pre- to mature B, not on plasma CD20 2H7 14-0209 Pre- to mature B, not on plasma
.: +33-4-77-81-4366; fax: +33-4-77-80-8294.Manuscript received 11 May 2003 Accepted 20 July 2003;Article OutlineAbstractIntroductionMaterials and methodsCell preparationsCell stimulationDetection of NFB translocation by flow cytometric assay in CD19+ B
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