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- 文献和实验
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- 供应商:
嵘崴达
- 库存:
大量
- 英文名:
Cholesterol Esterase Modified
- 保存条件:
见说明书
- 保质期:
见说明书
from Pseudomonas species, lyophilizate
Hydrolase that splits fatty acids from sterols.
Application
Use Cholesterol Esterase, chemically modified in diagnostic tests for the deter-
mination of cholesterol in combination with Cholesterol Oxidase, Catalog Nos.
11 479 709 103, 10 634 522 103 or 10 129 054.
Benefits
Take advantage of the enhanced stability of this enzyme in liquid re- ■
agents.
Rely on the proven diagnostic quality of this product. ■
EC 3.1.1.13
Properties
Nomenclature: Sterol-ester acylhydrolase
Molecular weight: ~129 kD
Isoelectric point: 4.5
Michaelis constant (Phosphate buffer, pH 7.5):
Cholesterol oleate: 7 x 10 -5 mol/l
Inhibitors: Heavy metals such as Cu 2+ , Ag + , Zn 2+
Activators: Detergents
pH optimum: 6.0-8.0; (maximum at pH 7.6) (see figure)
Temperature dependence: Not possible to determine under assay conditions
due to turbidity of Thesit at temperatures above +27°C.
pH stability: 6.0-6.5 (see figure)
Thermal stability: Below +20°C (see figure)
Specificity: Cholesterol esterase is an enzyme of lipid metabolism and gives
complete
cleavage of all serum cholesterol esters.
Specification
Appearance: Brownish lyophilizate
Solubility: Clear, brown solution in water (c=50 mg/ml)
pH value (c=50 mg/ml, in water): 7.0-8.0
Activity (+25°C, cholesterol oleate): ≥10 U/mg lyophilizate
Specific Activity: ≥100 U/mg protein
Volume Activity: For information only
Contaminants (expressed as percentage of Cholesterol Esterase activity):
ATPase: ≤0.005
Catalase: ≤1.00
Glycerokinase: ≤0.001
Hexokinase: ≤0.005
"NADH oxidase": ≤0.001
Uricase: ≤0.005
NaCl: 3±0.2 mol/l
Stability: At +2 to +8°C within specification range for 12 months. Store dry.
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文献和实验是怎么个情况还有人记得么?是不是先要乳化啊?什么叫乳化?自己想吧,乳化主要在小肠内,水解也在小肠内,胰液中至少有三种水解酶可以水解脂肪,胰脂肪酶、胰磷脂酶、胆固醇酯酶。三酶协同,脂肪呗胰脂肪酶分解为脂肪酸和甘油,卵磷脂呗胰腺脂酶A水解产生溶血卵磷脂酸和脂肪酸,胆固醇被胰胆固醇酶水解为游离的胆固醇和脂肪酸。 还有许多其他的营养物质也是被小肠全程吸收的,比如小肠近侧段主要吸收脂肪酸、一酰甘油、部分单糖、铁、钙、维生素(除B12外);中段吸收大部分氨基酸、部分单糖。回肠吸收胆盐和维生
问:请教肝酯酶的生理功能及其测定方法?答:肝脂酶(hepaticlipase或hepatiltriglyceridase或hepaticendothelaillipase,HL或HTGL)属于与血液循环中内源性TG代谢有关的酶之一,与LPL在功能上有相似之处,然而却是两种不同性质的酶。其特点是:①HL活性不需要ApoCⅡ作为激活剂;②SDS可抑制HL活性,而不受高盐浓度及鱼精蛋白的抑制;③主要作用于小颗粒脂蛋白,如VLDL、残粒残余CM及HDL,同时又调节胆固醇从周围组织转运到肝,使肝内
脂类乳化,使不溶于水的脂类分散成水包油的小胶体颗粒,提高溶解度增加了酶与脂类的接触面积,有利于脂类的消化及吸收。在形成的水油界面上,分泌入小肠的胰液中包含的酶类,开始对食物中的脂类进行消化,这些酶包括胰脂肪酶(pancreatic lipase),辅脂酶(colipase),胆固醇酯酶(pancreatic cholesteryl ester hydrolase or cholesterol esterase)和磷脂酶A2(phospholipase A2)。 食物中的脂肪
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