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罗氏原料10262595103
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Chol. Oxidase,Nocardia erythropolis
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Cholesterol Oxidasefrom Nocardia erythropolis, solutionOxidoreductase that catalyzes the interconversion of cholesterol to cholest-4-en-3-one.ApplicationUse Cholesterol Oxidase in diagnostic tests for the determination of choles-terol in combination with Cholesterol Esterase, Catalog Nos. 10 129 046 103, 10262 609 103, 11 015 923 103, 11 520 857 103 or 11 641 735 103.BenefitsApply this ready-to-use enzyme directly in your diagnostic test. ■Rely on the proven diagnostic quality of this product. ■EC 1.1.3.6PropertiesNomenclature: Cholesterol:oxygen oxidoreductaseMolecular weight: ~59 kDIsoelectric point: 4.85Michaelis constants (Cholesterol):Phosphate buffer, pH 7.0: 1 x 10 -6 mol/lTriton X-100/isopropanol, pH 7.0: 7 x 10 -6 mol/lInhibitors: ZnCl 2 , Brij 35, Tween 40, Tween 60, Hg 2+ , GSH, sodium dodecylsulfate,laurylbenzenesulfonateActivators: Hydroxypolyethoxydodecane, Triton X-100, DOCpH optimum: 7.0-8.0 (see figure)Temperature dependence: See figurepH stability: 5.5-6.5 (see figure)Thermal stability: Up to +50°C (see figure)Specificity: Cholesterol (100%), b-cholestanol, pregnenolone, b-sitosterol andstigmasterol react as substrates (65-70%). Dehydroisoandrosterone and ergos-terol show 5% relative activity. Androsterone, testosterone, β-estradiol, vitaminD3, cholic acid and cholesterol acetate do not react.SpecificationAppearance: Brownish-yellow, slightly turbid solution in NaCl solution, 3 mol/lpH value: 5.7-6.3Specific Activity: 25 U/mg proteinProtein: 1±0.1 mg/mlNaCl (chloride meter): 3±0.2 mol/lContaminants (expressed as percentage of Cholesterol Oxidase activity):Cholesterol esterase: ≤0.005Glucose oxidase: ≤0.01"NADH oxidase": ≤0.01Uricase: ≤0.01Stability: At +2 to +8°C within specification range for 12 months.罗氏工业原料
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