Blank control: K562.
Primary Antibody (green line): Rabbit Anti-CD32 antibody (bs-2573R-FITC)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
- ￥2980
- Bioss
- bs-2573R-FITC
- 2025年10月15日
- 产品信息以Bioss网站为准
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- 详细信息
- 技术资料
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产品信息以Bioss网站为准
- 规格：
100ul
| 产品编号 | bs-2573R-FITC |
| 英文名称 | CD32 Rabbit pAb, FITC conjugated |
| 中文名称 | FITC标记的低亲和力免疫球蛋白γFc区受体II-a/Fc γ RII a抗体 |
| 英文别名 | CD 32; CD32A; CD32B; CD32C; CDw32; Fc gamma RII; low affinity II receptor; Fc gamma receptor II; Fc-GAMMA-RII; Fc[g]RII; IgG Fc receptor II; FCG 2; FCG2; FcgammaRII; FCGR 2; FcGR; FCGR2; FCGR2A; FCGR2A1; FCGR2B; FCGR2C; FcgRII; Fcr-2; Fcr-3; FcRII a; FCRII; FcRII b; FcRII c; FcRIIC; IGFR 2; IGFR2; Ly-17; Ly-m20; LyM-1; Lymphocyte antigen 17; MGC23887; MGC30032; FCG2C_HUMAN. |
| 产品应用 | Flow-Cyt=0.2μg/Test Not yet tested in other applications. |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Fc gamma RII a |
| 亚型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Immunology > Cell Type Markers > CD > Non-lineage Stem Cells > Hematopoietic Progenitors > Myeloid > Neutrophil Lineage |
| 亚基 | Isoform IIB1 interacts with measles virus N protein. N protein is released in the blood following lysis of measles infected cells. This interaction presumably block inflammatory immune response. Interacts with INPP5D/SHIP1. Interacts with FGR. Interacts with LYN. |
| 亚细胞定位 | Cell membrane; Single-pass type I membrane protein. |
| 组织特异性 | Is the most broadly distributed Fc-gamma-receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells). Not detected in natural killer cells. |
| 翻译后修饰 | Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK. |
| 相似性 | Contains 2 Ig-like C2-type (immunoglobulin-like) domains. |
| 功能 | Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens. |
| 保存条件 | Shipped at 4℃. Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene encodes one member of a family of immunoglobulin Fc receptor genes found on the surface of many immune response cells. The protein encoded by this gene is a cell surface receptor found on phagocytic cells such as macrophages and neutrophils, and is involved in the process of phagocytosis and clearing of immune complexes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008] |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {0.2μg/Test} |
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CD32 Rabbit pAb, FITC conjugated(bs-2573R-FITC)-100ul
￥2980
