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phospho-PFKM/PFK1 (Ser775) Rab

bit pAb(bs-16457R)-50ul/100ul/200ul
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  • bs-16457R
  • 2025年10月24日
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-16457R
    英文名称phospho-PFKM/PFK1 (Ser775) Rabbit pAb
    中文名称磷酸化肌肉型6磷酸果糖激酶/磷酸果糖激酶1抗体
    英文别名PFKM(phospho S775); PFKM(phospho Ser775); p-PFKM(Ser775); Fructose 6 Phosphate Kinase; 6 phosphofructokinase Muscle Type; GSD7; PFKA; PFK-A; PFKP; PFKX; phosphofructo 1 Kinase Isozyme A; phosphofructo-1-kinase isozyme A; phosphofructokinase 1; phosphofructokinase M; phosphofructokinase-M; phosphofructokinase, muscle; phosphofructokinase, muscle type; phosphofructokinase, polypeptide X; phosphohexokinase; PFKAM_HUMAN; 6-phosphofructokinase, muscle type; PFK1; PFK-1; ATP-PFK; PPP1R122.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Rat (Mouse, Pig, Cow, Horse, Sheep)
    抗体来源Rabbit
    免疫原KLH conjugated Synthesised phosphopeptide derived from human PFKM around the phosphorylation site of Ser775
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量86 kDa
    浓度1mg/ml
    储存液Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4.
    研究领域

    Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of carbohydrates

    Metabolism > Pathways and Processes > Metabolic signaling pathways > Carbohydrate metabolism

    Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Energy Metabolism

    Signal Transduction > Metabolism > Energy Metabolism

    亚基Homo- and heterotetramers. Muscle is M4, liver is L4, and red cell is M3L, M2L2, or ML3. Interacts (via C-terminus) with HK1 (via N-terminal spermatogenic cell-specific region)
    亚细胞定位Cytoplasm.
    翻译后修饰GlcNAcylation decreases enzyme activity.
    相似性Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily.
    功能Catalyzes the third step of glycolysis, the phosphorylation of fructose-6-phosphate (F6P) by ATP to generate fructose-1,6-bisphosphate (FBP) and ADP.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009]

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    Tissue/cell: Rat pancreas tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-phospho-PFKL (Ser775) Polyclonal Antibody, Unconjugated(bs-16457) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    产品细节图片2
    Sample:
    Lane 1: Human A431 cell lysates
    Lane 2: Human HeLa cell lysates
    Lane 3: Human Raji cell lysates
    Lane 4: Human SH-SY5Y cell lysates
    Lane 5: Human LOVO cell lysates
    Lane 6: Human MCF-7 cell lysates
    Primary: Anti-phospho-PFKM/PFK1 (Ser775) (bs-16457R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 86 kDa
    Observed band size: 77 kDa

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