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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
嵘崴达
- 保存条件:
见说明书
- 保质期:
见说明书
- 库存:
需确认
- 英文名:
DRYSTRIP COVER FLUID, 1000 ML
- 规格:
1000ml
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| 17133501 | DRYSTRIP COVER FLUID 1000 ML |
| 17600086 | IPG-BUFFER PH 4-7L |
| 17600087 | IPG-BUFFER PH 3-10L |
| 17600088 | IPG-BUFFER PH 3-10NL |
| 17600110 | IMMOBILINE DRYSTRIP PH 4-7 7CM |
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文献和实验2-D Polyacrylamide Gel Electrophoresis
on top of 50 ml DryStrip Cover fluid . 3.Remove strips. 4.Place on Whatman paper gel-side up. TIP: Placing the strips gel-side down might result in protein loss and gel damage. 5.Leave for ~1 min. 6.Place into the electrophoresis chamber gel
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
culture fluid, inoculate a single or multiple 96 deep-well blocks containing 1.2-1.6 ml per well of LB media with appropriate antibiotic. Alternatively, 24 deep-well or 48 deep-well blocks may be used with up to 4.8 or 2.4ml of media respectively. Cover
at 1000 rpm Remove supernatant e.g. with a cell culture pipettor until 5 ml remain Gently add 40 ml KCl (0.075 M, 37°C), first 5 ml drop by drop (hypotonic treatment) Incubate for 25 min in 37°C water bath Centrifuge 10 min at 1000
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