4673409001/FastStart TaqMan® 探

FastStart TaqMan^® 探针预混剂

FastStart TaqMan^® Probe Master

General description

FastStart TaqMan^® Probe Master is a ready-to-use hot start reaction mix without ROX for quantitative polymerase chain reaction (qPCR) and reverse transcription (RT)-qPCR on real-time PCR systems other than the LightCycle^® instruments. It is a 2x concentrated master mix that contains all the reagents (except primers, probe, and template). This master mix simplifies the preparation of reactions for DNA detection and analysis. It allows very sensitive detection and quantification of defined DNA sequences.
Hot start protocols have been also been shown to significantly improve the specificity and yield of PCR. Heat-labile blocking groups on some of the amino acid residues of FastStart Taq DNA Polymerase make the modified enzyme inactive at room temperature. Therefore, there is no elongation during the period when primers can non-specifically bind. The FastStart Taq DNA Polymerase is activated by removing the blocking groups at a high temperature (i.e., a pre-incubation step at +95°C).
This FastStart TaqMan^® Probe Master can be used for the amplification and detection of any DNA or cDNA target, including those that are GC- or AT-rich. However, you would need to adapt your detection protocol to the reaction conditions of the particular real-time PCR instrument used and design a specific hydrolysis probe and PCR primers for each target.
Combination of this master mix with our Transcriptor First Strand cDNA Synthesis Kit achieves excellent results in two-step qRT-PCR.

热启动协议已被证明能显著提高PCR的特异性、灵敏度和产量。快速启动Taq DNA聚合酶的某些氨基酸残基上的不耐热保护基使修饰酶在室温下无活性。因此，在引物可以非特异性结合的时期没有延伸。快速启动Taq DNA聚合酶通过在高温条件下除去保护基来激活（即+95°C条件下的预保温步骤）。

Features and Benefits

• 提高 qPCR 灵敏度和特异性：
热启动酶 FastStart Taq DNA 聚合酶和主混合物中包含的纯度核苷酸产生较低的循环阈值 (C _t )。
• 用于任何基于探针的检测：
使用通用探针库探针或任何其他水解探针的检测中获得灵敏、特异结果。
• 扩增和检测广泛的DNA或cDNA靶：
扩增长度高达500 bp的片段，包括那些富含GC或AT的片段。
• 使用除 LightCycler^®仪器以外的任何实时 qPCR 仪器：
从两种制剂中选择——一种含有 ROX 引用染料，另一种不含 ROX。
• 在琼脂糖凝胶上显示扩增产物。