- ￥3526
- roche
- 10650129001
- 2025年11月20日
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- 详细信息
- 技术资料
- 库存：
需确认
- 保质期：
见产品外包装
- CAS号：
见产品外包装
- 供应商：
嵘崴达
- 英文名：
Pvu I from Proteus vulgaris
- 保存条件：
−20°C
- 规格：
500 units
Pvu I
from Proteus vulgaris
| packaging | pkg of 100 U (10650137001 [5 U/μl]) |
| pkg of 500 U (10650129001 [5 U/μl]) |
Specificity
Pvu I recognizes the sequence CG°AT↓ *CG and generates fragments with 3′-cohesive termini.
Recognition sites: CG°AT*CG
CG°AT*CG
Restriction site: CG°AT↓*CG
CG°AT↓*CG
Heat inactivation: No inactivation of Pvu I after incubation at 65 °C for 15 minutes.
Quality
Absence of nonspecific endonuclease activities
1μg λDNA is incubated for 16hours in 50μl SuRE/Cut Buffer H with an excess of Pvu I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis.
Absence of exonuclease activity
Approximately 5μg [3H] labeled calf thymus DNA are incubated with 3μl Pvu I for 4hours at +37°C in a total volume of 100μl 50mM Tris-HCl, 10mM MgCl2, 1mM Dithioerythritol, pH approximately 7.5. Under these conditions, no release of radioactivity is detectable, as stated in the certificate of analysis.
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