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- 详细信息
- 文献和实验
- 技术资料
- 库存:
需确认
- 保质期:
见产品外包装
- CAS号:
见产品外包装
- 供应商:
嵘崴达
- 英文名:
Pvu I from Proteus vulgaris
- 保存条件:
−20°C
- 规格:
500 units
Pvu I
from Proteus vulgaris
| packaging | pkg of 100 U (10650137001 [5 U/μl]) |
| pkg of 500 U (10650129001 [5 U/μl]) |
Specificity
Pvu I recognizes the sequence CG°AT↓ *CG and generates fragments with 3′-cohesive termini.
Recognition sites: CG°AT*CG
CG°AT*CG
Restriction site: CG°AT↓*CG
CG°AT↓*CG
Heat inactivation: No inactivation of Pvu I after incubation at 65 °C for 15 minutes.
Quality
Absence of nonspecific endonuclease activities
1μg λDNA is incubated for 16hours in 50μl SuRE/Cut Buffer H with an excess of Pvu I. The number of enzyme units which do not change the enzyme-specific pattern is stated in the certificate of analysis.
Absence of exonuclease activity
Approximately 5μg [3H] labeled calf thymus DNA are incubated with 3μl Pvu I for 4hours at +37°C in a total volume of 100μl 50mM Tris-HCl, 10mM MgCl2, 1mM Dithioerythritol, pH approximately 7.5. Under these conditions, no release of radioactivity is detectable, as stated in the certificate of analysis.
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文献和实验endonucleases)和连接酶(ligases)。 限制性内切酶能够识别DNA分子上的特定核苷酸序列,并在该处特异性切断DNA分子。例如,Pvu I(细菌Proteus vulgaris 分离)只识别和切断6核苷酸序列CGATCG;从相同细菌分离的Pvu II,却只识别并切断CAGCTG。许多限制性内切酶的识别位点是6个核苷酸,但是,也有识别4个或5个、甚至8个核苷酸顺序的限制性内切酶。此外,有些限制性内切酶的识别顺序可能不是唯一的,例如,Hinf I可以识别并切断GAATC、GATTC
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