重组Ecotin怎么卖

特别声明：本产品及我公司所售其他产品均为科研类试剂产品，严禁用于药物、医疗及其他非科研用途。

北京百奥莱博主要从事生物试剂的生产和销*(代"售")，产品线涵盖重组Ecotin怎么卖在内的分子生物学试剂产品，还包括PCR及荧光定量PCR、核酸提取试剂盒类、样本核酸保护试剂、核酸提取、克隆与转化、蛋白纯化、蛋白检测、免疫组化、细胞凋亡与抗体等。

名称：重组Ecotin怎么卖
英文名：Recombinant E. coli Ecotin
规格：10μg|50μg|500μg|1mg
产地：国产|进口
品牌：百奥莱博
编号：JN1657
本品由我们的大肠杆菌表达系统制备而成，目标蛋白包含序列(Met1-Arg162)在C端带有His标签。

Ecotin质量控制：＞95%(SEC-HPLC，还原性SDS-PAGE)


Ecotin制剂：冻干品

Ecotin保存：
冻干蛋白长期保存应置于-20℃以下，常温可稳定保存3周。
复溶蛋白溶液可在4℃保存2～7天。
复溶后的蛋白溶液应尽量避免反复冻融，可根据使用量分装冻存在-20℃以下长达3个月。

Ecotin复溶：
打开试剂管前请先离心。
复溶浓度推荐大于100 μg/ml。
Dissolve the lyophilized protein in 1X PBS.
复溶后，请根据用量分装冻存，避免反复冻融。

关于Ecotin：
Ecotins are dimeric periplasmic proteins from Escherichia coli and related Gram-negative bacteria that have been shown to be potent and general inhibitors of many trypsin-fold serine proteases of widely varying substrate specificity, which belong to MEROPS peptidase family S1. Ecotin protein inhibits chymotrypsin, trypsin, elastases, factor X, kallikrein as well as a variety of other proteases and has been characterized as an extremely potent anticoagulant and reversible tight-binding inhibitor of human factor Xa (FXa). The power of inhibition is not linked to specific protease specificity. Immobilized Ecotin has been used to affinity-purify recombinant trypsinogen, indicating that it may also be used to purify additional serine protease zymogens. Comp*(代"a")red to other serine protease inhibitors such as members of the serpin family, the reactive site of ecotin is Met104 (P1). Phylogenetic analysis suggested that Ecotin has an exogenous target, possibly neutrophil elastase. Ecotin from E. coli, Yersinia pestis, and Pseudomonas aeruginosa, all species that encounter the mammalian immune system, inhibit neutrophil elastase strongly while ecotin from the plant pathogen Pantoea citrea inhibits neutrophil elastase 1000-fold less potently. Ecotins all potently inhibit pancreatic digestive peptidases trypsin and chymotrypsin, while showing more variable inhibition of the blood peptidases Factor Xa, thrombin, and urokinase-type plasminogen activator. Ecotin is synthesized as a 162 amino acid precursor with a 20 amino acid signal peptide necessary to direct it to the periplasmic space. The mature protein (residues 21 - 162) is expressed and purified.

我公司的重组Ecotin怎么卖，性价比高，货期短，送货上门，欢迎您来电咨询购买，另外，我公司还生产供应销*(代"售")下列产品：

·双链DNA染料(SYBR Green)
编号：JN0062
英文名称：SYBR Green I
规格：0.5ml×5
  本品是一种直接与双链DNA结合的荧光染料，是荧光定量PCR最常用的DNA结合染料。在定量PCR中，SYBR Green I可与dsDNA非特异结合后发出荧光。可以通过检测反应体系中的荧光强度达到检测PCR产物扩增量的目的。SYBR Green I在游离状态下发出微弱的荧光，一旦与dsDNA结合，其荧光强度增加1000倍。一个反应发出的荧光信号与出现的dsDNA量成正比，且会随着扩增产物的增加而增加，通过检测PCR反应液中的荧光信号强度，可以对目的基因进行准确定量，同时还可以测定扩增的目的DNA片段的熔解温度。

使用建议：
使用浓度对荧光PCR结果的影响；
SYBR Green I的使用浓度是荧光定量PCR实验成功的关键因素。SYBR Green I的浓度过低会使荧光信号的变化降低，这就意味着低拷贝的样品可能无法检出；而SYBR Green I的浓度过高会抑制PCR反应，降低PCR反应效率。所以一般在使用SYBR Green I时应根据实际情况优化使用浓度，反应的终浓度为0.2×到1×之间。
Mg2+浓度的影响；
提高Mg2+浓度可以降低SYBR Green I对PCR反应的抑制作用。我们建议用SYBR Green I进行荧光定量PCR反应时，Mg2+浓度应比无SYBR Green I 的普通PCR反应高出0.5到3mM。

储存条件：-20℃


重组Ecotin怎么卖关键词：重组Ecotin蛋白,Recombinant E. coli Ecotin,百奥莱博,JN1657


·重组人白介素IL-1β(白细胞介素1β)
编号：JN0395
英文名称：Recombinant Human Interleukin-1 beta/Catabolin
规格：10μg|50μg|500μg|1mg
本品由我们的大肠杆菌表达系统制备，表达蛋白包含序列(Ala117-Ser269)。

IL-1B质量控制：＞95%(SEC-HPLC，还原性SDS-PAGE)

IL-1B制剂：冻干品

IL-1B保存：

关于IL-1B：
IL1B is a 269 amino acids protein which belongs to the IL-1 family. Interleukin 1 (IL-1) is a family of polypeptide cytokines consisting of two agonists, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2) encoded by two distinct genes and perform identical biological functions. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response. It is identified as endogenous pyrogens, and is reported to stimulate the release of prostaglandin and collagenase from synovial cells.


重组Ecotin怎么卖关键词：重组Ecotin蛋白,Recombinant E. coli Ecotin,百奥莱博,JN1657


·重组人真核翻译起始因子4E结合蛋白2(EIF4EBP2)
编号：JN0905
英文名称：Recombinant Human Eukaryotic Translation Initiation Factor 4E-Binding Protein 2
规格：10μg|50μg|500μg|1mg
本品由我们的大肠杆菌表达系统制备而成，目的基因编码的Met1-Ile120在N端含有His标签。

EIF4EBP2质量控制：＞95%(还原性SDS-PAGE)

EIF4EBP2制剂：冻干品

EIF4EBP2保存：
冻干蛋白置于-20℃以下可长期保存，室温条件下可稳定保存3周。
复溶蛋白溶液可在4～7℃保存2～7天，可分装后置于-20℃保存三个月。

EIF4EBP2复溶：
打开试剂管前请先离心。
复溶浓度推荐大于100 μg/ml。
冻干蛋白请溶于ddH2O。
复溶后，请根据用量分装冻存，避免反复冻融。

关于EIF4EBP2：
Eukaryotic Translation Initiation Factor 4E-Binding Protein 2 (EIF4EBP2) is a member of the Eukaryotic Translation Initiation Factor 4E Binding Protein Family. EIF4EBP2 regulates eIF4E activity by preventing its assembly into the eIF4F complex, mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase pathway. This regulation of is associated to cell proliferation, cell differentiation and viral infection. Phosphorylated EIF4EBP2 on serine and threonine residues in response to insulin, EGF and PDGF.

我公司强烈推荐重组蛋白系列产品，热情期待您的咨询选购重组Ecotin怎么卖。