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CD20 antibody

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  • ¥780 - 2200
  • 康朗生物
  • 中国/美国/德国
  • 2025年07月10日
  • ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500
  • Rabbit
  • Human, Mouse, Rat, Dog, Cow, Rabbit, 
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上海康朗生物科技有限公司
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    • 供应商：

      上海康朗生物科技有限公司

    • 库存：

      大量

    • 目录编号：

      kl-0080R

    • 克隆性：

      多克隆

    • 抗原来源：

      Rabbit

    • 保质期：

      12个月

    • 抗体英文名：

      CD20 antibody

    • 抗体名：

      CD20 antibody

    • 宿主：

      Rabbit

    • 适应物种：

      Human, Mouse, Rat, Dog, Cow, Rabbit, 

    • 免疫原：

      KLH conjugated synthetic peptide derived from human Nestin:801-900/1621

    • 亚型：

      IgG

    • 形态：

      冻干粉或液体

    • 应用范围：

      ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500

    • 浓度：

      1mg/ml

    • 保存条件：

      -20 °C

    • 规格：

      50ul 100ul 200ul

    CD20 antibody
    中文名称 CD20抗体
    别    名 CD20_HUMAN; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1; Bp35; Leukocyte surface antigen Leu-16; Membrane-spanning 4-domains subfamily A member 1; MS4A1; MS4A2; B1; CVID5; LEU-16; S7.  
    规格价格 50ul/780元 购买    100ul/1380元 购买    200ul/2200元 购买    大包装/询价
    说 明 书 50ul  100ul  200ul
    研究领域 肿瘤  免疫学  干细胞  细胞表面分子  细胞类型标志物  淋巴细胞  b-淋巴细胞  
    抗体来源 Rabbit
    克隆类型 Polyclonal
    交叉反应 Human, Mouse, Rat, Dog, Cow, Rabbit, 
    产品应用 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1μg /test IF=1:100-500 （石蜡切片需做抗原修复） 
    not yet tested in other applications.
    optimal dilutions/concentrations should be determined by the end user.
    分 子 量 33kDa
    细胞定位 细胞膜 
    性    状 Lyophilized or Liquid
    浓    度 1mg/ml
    免 疫 原 KLH conjugated synthetic peptide derived from human CD20:133-182/297 <Cytoplasmic>
    亚    型 IgG
    纯化方法 affinity purified by Protein A
    储 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
    保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
    PubMed PubMed
    产品介绍 background:
    CD20 is a non glycosylated protein with a molecular weight of 35 or 37 kDa depending on the degree of phosphorylation. Although not a member of the tetraspanin superfamily of cell surface receptors, it crosses the cell membrane four times. The CD20 antigen is present on human pre B lymphocytes and on B lymphocytes at all stages of maturation, except on plasma cells. Low level expression of the CD20 antigen has been detected on normal T lymphocytes. The CD20 molecule is involved in regulation of B cell differentiation, presumably via its reported function as a Ca++ channel subunit. 

    Function:
    This protein may be involved in the regulation of B-cell activation and proliferation

    Subcellular Location:
    Membrane; Multi-pass membrane protein.

    Tissue Specificity:
    Expressed on B-cells.

    Post-translational modifications:
    Phosphorylated. Might be functionally regulated by protein kinase(s).

    DISEASE:
    Defects in MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5) [MIM:613495]; also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen. The defect results from a failure of B-cell differentiation and impaired secretion of immunoglobulins; the numbers of circulating B-cells is usually in the normal range, but can be low.

    Similarity:
    Belongs to the MS4A family.

    SWISS:
    P11836

    Gene ID:
    931

    Database links:

    Entrez Gene: 931 Human

    Omim: 112210 Human

    SwissProt: P11836 Human

    Unigene: 712553 Human



    Important Note:
    This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

    [PHARMACEUTICAL] Monoclonal antibodies (mAb) against CD20 are used to treat B-cell non-Hodgkin lymphoma (NHL). These antibodies include Rituximab (Mabthera), Britumomab (Zevalin) and Tositumomab (Bexxar). CD20 engaged by mAb can generate transmembrane signals capable of directly controlling cell growth and triggering cell death in certain tumors. Alternatively, mAb can mediate complement-dependent cytotoxicity. 

    B淋巴细胞标志物

    CD20是B细胞特异性抗原，其表达呈细胞类型一种质膜蛋白质，参与信号传导作用，此抗体识别大多数B细胞上的一种抗原。多用于标记B淋巴细胞及B细胞淋巴瘤。
    CD20一般不与T细胞交叉反应，对B细胞淋巴瘤和急慢性淋巴细胞白血病等有较好的细胞特异性，因此在恶性淋巴瘤，尤其是T或B细胞淋巴瘤的分类上，CD20是最常用的B细胞标志。
    产品图片 产品细节图片1
    Tissue/cell: human laryngo carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; 
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
    Incubation: Anti-CD20 Polyclonal Antibody, Unconjugated(bs-0080R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    产品细节图片2
    Tissue/cell: rat colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; 
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; 
    Incubation: Anti-CD20 Polyclonal Antibody, Unconjugated(bs-0080R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

    产品细节图片3
    Blank control: U937 (blue). 
    Primary Antibody:Rabbit Anti- CD20 antibody(bs-0080R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; 
    Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions ); 
    Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
    Protocol
    The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody (bs-0080R, 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

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