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- 详细信息
- 文献和实验
- 技术资料
- 库存:
大量
- 英文名:
Recombinant SAK
- 保质期:
一年
- 供应商:
上海联迈生物工程有限公司
- 保存条件:
-20℃或更低温度保存
- 规格:
1mg
| 产品编号 | 产品名称 | 产品包装 | 产品价格 |
| LM7050-1mg | Recombinant SAK | 1mg | 4553.00元 |
| Species | Gene ID | Accession | Source | Length | MW | Tag |
| - | 3921865 | LM2FWV3 | E. coli | 136aa | 15.6kDa | - |
| About this Protein | |
| Name | Recombinant SAK (Recombinant Staphylokinase; rSAK);重组葡激酶 |
| Synonyms | Staphylokinase, putative; SAOUHSC_02171 |
| Purity | >97% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The specific activity determined by fibrining lysis in agarose plate is 5.0×104IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4. |
| Endotoxin | Less than 1EU/μg of rSAK as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Enzymes |
| Background | Staphylokinase is an amino acid enzyme secreted by several species of streptococci. It is a 16kDa potent plasminogen activator that converts plasminogen into plasmin which can digest fibrin the major constituent of blood thrombi. SAK forms 1:1 complex with plasmin, which is a positive feedback of producing other complexes. Recent studies on the thrombolytic potential of recombinant SAK in achieving early perfusion in myocardial infarction and in the dissolution of platelet-rich clot have clearly established its immense utility in clinical medicine as a thrombolytic agent and suggested that it can be developed as a potent clot-dissolving agent. |
| Amino Acid Sequence | SSSFDKGKYK KGDDASYFEP TGPYLMVNVT GVDGKRNELL SPRYVEFPIK PGTTLTKEKI EYYVEWALDA TAYKEFRVVE LDPSAKIEVT YYDKNKKKEE TKSFPITEKG FVVPDLSEHI KNPGFNLITK VVIEKK |
| 产品编号 | 产品名称 | 包装 |
| LM7050-20μg | Recombinant SAK | 20μg |
| LM7050-100μg | Recombinant SAK | 100μg |
| LM7050-1mg | Recombinant SAK | 1mg |
| - | 说明书 | 1份 |
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
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