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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 库存:
现货
- 英文名:
Hanks’ Balanced Salt solution
- 供应商:
上海善然生物科技有限公司
- 保存条件:
低温
Other Notes
Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when growing cells at low densities.
Application
Calcium and magnesium support cell adhesion. Used to minimize cell attachment or aggregation.
Hanks’ Balanced Salt solution has been used:
• to study the degradation and cytotoxic effects of the composite material and the ion and/or magnetite release from the scaffolds.[3]
• to measure the stability of the magnetic scaffold material in bone tissue engineering.[4]
• in Fluorometric Measurements of intracellular calcium levels [Ca2+][5]
• for isolating polymorphonuclear leukocytes (PMN) from umbilical cord blood[2]
• For assessing airway inflammation in mice[1]
General description
The essential function of Hanks′ Balanced Salt solution is to maintain pH as well as osmotic balance. It also provides water and essential inorganic ions to cells.
性质
Related Categories Balanced Salt Mixtures and Solutions, Buffered Salt Solutions, Cell Culture Media, Core Bioreagents, Hank’s Balanced Salts,
更多...
Quality Level PREMIUM
sterility sterile-filtered
form liquid
impurities endotoxin, tested
suitability suitable for cell culture
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文献和实验1.培养用水:体外培养的细胞对水质特别敏感,对水的纯度要求较高。培养用水中如果含有一些杂质,即使含量极微,有时也会影响细胞的存活和生长,甚至导致细胞死亡。用金属蒸馏器制备的蒸馏水,可能会含有某些金属离子,一般不作为培养用水。配制培养用液应使用经石英玻璃蒸馏器三次蒸馏的三蒸水或超纯水净化装置制备的超纯水。最好用龙头瓶贮存,存放时间一般不应超过2周。 2.缓冲溶液、生理盐水、平衡盐溶液
配方: 氯化钠:8g 氯化钾:0.4g 葡萄糖:1g 磷酸二氢钾:60mg 磷酸氢二钠:47.5mg ddH2O定容至1L 调pH至7.2; 本实验室配方: Hank's平衡盐成品:9.5g 碳酸氢钠:0.35g ddH2O定容至1L 分装
动物细胞培养中Hanks液、D-Hanks液和消化液的配制方法
相关专题 Hanks液可以维系细胞正常渗透压与pH,本文主要记录了动物细胞培养 中Hanks液、D-Hanks液和消化液(胰酶液)的配制方法及其注意事项。 动物细胞培养: Hanks、D-Hanks液和消化液(胰酶液)的配制 实验目的: 熟练掌握Hanks、D-Hanks液等平衡盐溶液的配制,了解消化液(胰酶液)的配制方法。 实验原理:Hanks液是常见的平衡盐溶液(BSS)之一。D-Hanks液
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