热销供应Roche货号10105031001现货β-半乳糖苷

热销供应Roche货号10105031001现货β-半乳糖苷酶Merck-Millipore-Sigma-Roche官方正式授权代理商19121878899上海睿安生物科技有限公司

Roche货号10105031001，(规格1500 units/瓶)╳目录单价¥1105.82元/瓶

β-半乳糖苷酶

from E.coli overproducer

别名:β-半乳糖苷酶

说明

General description

ββ-半乳糖苷酶或β-D-半乳糖苷半乳糖水解酶是由4条相同的各含1023个氨基酸的肽链组成的四聚体。对D-半乳糖表现高度专一性，完全激活需要K⁺或Na⁺和Mg²⁺。ββ-半乳糖苷酶催化含氧苷键的β-d-吡喃半乳糖苷的反应。

37℃，以2-硝(xiāo)基苯基-β-D-半乳糖苷为底物，25℃，以乳糖为底物，约30 U/mg；用BSA标准化。

Application

ββ-半乳糖苷酶已用于酶联免疫吸附测定(ELISA)。使用本β-半乳糖苷酶可制作酶测定的校准曲线。

Biochem/physiol Actions

切割单糖、寡糖或糖肽的1,4-连接末端半乳糖残基。

Physical form:悬浮于3.2M硫酸铵溶液中，pH约为6，晶体状

Preparation Note

硫酸铵制剂可以在2至8℃下稳定保存至标签注明的失效日期。可直接用于大多数应用，如乳糖的定量。

在不存在硫酸铵时，应使用Mg²⁺(89mM)和硫醇试剂(1mM β-巯基乙醇，1mM二硫苏糖醇)稳定β-半乳糖苷酶溶液[Beutler，1984]。硫醇可以减缓由分子内二硫键引起的酶二聚体形成。

活化剂:需要K(50mM)进行激活(乳糖水解)。
Na(50mM)也是一种活化剂，特别是对于2-硝(xiāo)基苯基-β-D-吡喃半乳糖苷的水解。

Analysis Note

污染物:GIDH、GPT、LDH、MDH和草酰乙酸脱羧酶，每种污染物含量均<0.01％

Other Notes:仅用于生命科学研究。不用于诊断操作。

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