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- EASYBIO
- BE7001-10
- 2025年12月07日
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His-HRP Mouse Monoclonal Antibody
A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.
Product Name: His-Tag Mouse Monoclonal Antibody-HRP conjugated
Isotype: IgG1
Clone Number: 6E8
Storage Buffer : PBS, pH 7.4, 50% Glycerol
Storage instructions: -20°C. Do not aliquot the antibody
Recommended dilutions: WB: 1:2,000-5,000
Optimal dilutions should be determined by the end user.
Specificity: The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins.
Form: Liquid
Reactivity: ALL
Western blot analysis of His recombinant protein with His Mouse mAb(6E8) HRP Conjugated diluted at
1）1:2,000
2）1:5,000
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文献和实验Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
Monoclonal Antibody Production
Mouse Immunization Order 6 six week old Balb/C mice and let the ARC know they are coming. Have your antigen ready for when they arrive. Once they get there earmark the mice and perform a pre-bleed on them to be used as an ELISA control
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
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