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- 详细信息
- 文献和实验
- 技术资料
- 库存:
582
- 英文名:
Recombinant Mouse Interferon gamma
- 保质期:
长期
- 供应商:
北京百奥莱博科技有限公司
- 保存条件:
冻干蛋白置于-20℃
特别声明:本产品及我公司所售其他产品均为科研类试剂产品,严禁用于药物、医疗及其他非科研用途。
重组小鼠干扰素IFN-γ(带His标签)厂家直销的品牌:百奥莱博,是优质的重组蛋白产品,用于生化科研试验,本产品质量好,且极具价格优势,深为用户称道,了解更多重组小鼠干扰素IFN-γ(带His标签)等重组蛋白产品请联*(代"系")我司咨询订购。
名称:重组小鼠干扰素IFN-γ(带His标签)厂家直销
规格:10μg|50μg|500μg|1mg
英文名:Recombinant Mouse Interferon gamma
品牌:百奥莱博
本品由我们的哺乳动物细胞表达系统制备而成,目的基因编码的His23-Cys155在C端含有His标签。
IFN-gamma/IFN-γ质量控制:>95%(还原性SDS-PAGE)
IFN-gamma/IFN-γ制剂:冻干品
IFN-gamma/IFN-γ保存:
冻干蛋白置于-20℃以下可长期保存,室温条件下可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天,可分装后置于-20℃保存三个月。
IFN-gamma/IFN-γ复溶:
打开试剂管前请先离心。
复溶浓度推荐大于100 μg/ml。
冻干蛋白请溶于ddH2O。
复溶后,请根据用量分装冻存,避免反复冻融。
关于IFN-gamma/IFN-γ:
Mouse Ifng is a secreted protein which belongs to the type I I (or gamma) interferon family. IFNG is produced by lymphocytes and activated by specific antigens or mitogens. In addition to having antiviral activity, IFNG also has important immunoregulatory functions. It is a potent activator of macrophages and has antiproliferative effects on transformed cells. It can potentiate the antiviral and antitumor effects of the type I interferons. Genetic variation in IFNG is associated with the risk of aplastic anemia (AA) which is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
关于重组小鼠干扰素IFN-γ(带His标签)厂家直销的更详细说明,请致电我公司咨询,我们将以最饱满的热情为您提供解答,此外,我公司专业供应下列产品:
·重组人Resistin(RETN)(脂肪组织特异性分泌因子)
编号:JN1355
英文名称:Recombinant Human Adipose tissue-specific secretory factor
规格:10μg|50μg|500μg|1mg
本品由我们的哺乳动物细胞表达系统制备而成,目的基因编码的Lys19-Pro108在C端带有Fc标签。
Resistin/RETN质量控制:>95%(还原性SDS-PAGE)
Resistin/RETN制剂:冻干品
Resistin/RETN保存:
冻干蛋白置于-20℃以下可长期保存,室温条件下可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天,可分装后置于-20℃保存三个月。
Resistin/RETN复溶:
打开试剂管前请先离心。
复溶浓度推荐大于100 μg/ml。
冻干蛋白请溶于ddH2O。
复溶后,请根据用量分装冻存,避免反复冻融。
关于Resistin/RETN:
Resistin known as adipose tissue-specific secretory factor (ADSF) or C/EBP-epsilon-regulated myeloid-specific secreted cysteine-rich protein (XCP1) that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. The length of the resistin pre-peptide in human is 108 amino acid residues and in the mouse and rat it is 114 aa; the molecular weight is ~12.5 kDa. Resistin is a cytokine whose physiologic role has been the subject of much controversy regarding its involvement with obesity and type II diabetes mellitus (T2DM). Resistin has been shown to cause "high levels of bad cholesterol (low-density lipoprotein or LDL), increasing the risk of heart disease, resistin increases the production of LDL in human liver cells and also degrades LDL receptors in the liver. Potentially links obesity to diabetes.
重组小鼠干扰素IFN-γ(带His标签)厂家直销关键词:带His标签,重组小鼠干扰素IFN-γ蛋白,重组干扰素IFN-γ,JN0432
·重组人白介素20受体α(IL-20Rα)
编号:JN0369
英文名称:Recombinant Human Interleukin-20 Receptor Subunit alpha
规格:10μg|50μg|500μg|1mg
本品由我们的哺乳动物表达系统制备,目的基因编码的Val30-Lys250在N端含有Fc标签。
IL-20RA质量控制:>95%(还原性SDS-PAGE)。
IL-20RA制剂:冻干品
IL-20RA保存:
冻干蛋白可在-20℃长期保存,室温也可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天。
复溶后建议分装冻存在-20℃,可保存3个月,应避免反复冻融。
IL-20RA复溶:
1、试剂开盖前请先离心。
2、复溶浓度应大于100 μg/ml.
3、冻干蛋白溶于ddH2O中
4、复溶后根据使用量分装保存,避免反复冻融。
关于IL-20RA:
Interleukin-20 Receptor Subunit α (IL20RA) is a single-pass type I membrane protein that is a member of the type II cytokine receptor family. IL20RA is synthetized a 553 amino acid glycoprotein precursor containing a 29 amino acid signal peptide, a 221 amino acid extracellular domain with two fibronectin type-III domains, a 24 amino acid transmembrane region, and a 279 amino acid intracellular domain. IL20RA is widely expressed with highest levels found in skin and testis and high levels in brain. IL20RA forms a heterodimer with IL20RB, and the complex serves as a receptor for IL19, IL20 and IL24. IL20RA also forms a heterodimer with the unique and specific receptor IL10RB and functions as the receptor for IL26.
重组小鼠干扰素IFN-γ(带His标签)厂家直销关键词:带His标签,重组小鼠干扰素IFN-γ蛋白,重组干扰素IFN-γ,JN0432
·重组人FAM3D蛋白
编号:JN0525
英文名称:Recombinant Human Protein FAM3D
规格:10μg|50μg|500μg|1mg
本品由我们的哺乳动物细胞表达系统制备而成,目的基因编码的Tyr26-Phe224在C端含有His标签。
FAM3D质量控制:>95%(还原性SDS-PAGE)
FAM3D制剂:液体
FAM3D保存:收到货后请置于-20℃,可保存6个月,避免反复冻融。
关于FAM3D:
Protein FAM3D is a novel cytokine-like protein that belongs to the FAM3 family. Human FAM3D is synthesized as a 224 amino acid precursor that contains a 25 amino acid signal sequence and a 199 amino acid mature chain. FAM3D is identified based on structural, but not sequence, homology to short chain cytokines including IL-2, IL-4 and GM-CSF. FAM3 proteins are four helix bundle cytokines with four conserved cysteines in all members (FAM3A-D). FAM3B is highly expressed in alpha and beta cells of the pancreas and is being investigated as a potential contributor to beta cell death and development of Type I Diabetes.
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文献和实验基因组 DNA 为模板进行 PCR 扩增,电泳确认 PCR 产物大小,对大小正确的产物进行测序,如测序结果与理论序列一致,则确认该基因编辑小鼠模型为正确重组模型。 TIPS 双臂 PCR 产物需测通:我们在实践中发现,即使 Donor 序列完全正确,部分鼠会存在突变或片段缺失的情况,我们推测是细胞在 Donor 重组前或过程中对 Donor 发生了编辑或重组后结构不稳定等原因所致,所以只对 Donor 各个部分接头测序是不严谨的,建议测通。 下面以 CKO 模型鉴定为例说明双臂 PCR 鉴定
,会采用两种转基因动物进行杂交,即一种带Cre的转基因动物和一种带loxP序列的转基因动物进行交配,使得后代既带Cre又带loxP基因,然后Cre重组酶会识别loxP位点,导致基因重组(图2)。由于Cre基因的表达受上游启动子的调控,这样启动子的时空特异性就决定了基因重组的时空特异性。虽然该方法可以实现高效、特异的基因重组,但是以下不足限制了用转基因动物进行基因操作的发展: 1) 转基因动物的难以获得:目前虽然已经有一些Cre和loxP转基因动物,但是要获得这些转基因小鼠比较困难,特别
【求助】一个关于基因改造的问题,希望各位站友帮帮忙!祝各位国庆快乐!
胚胎并并放到鼠子宫里培养成子代。这个基因(包括启动子)会通过基因重组的方式可以整合到小鼠胚胎基因组里面从而可以在子代中表达,这个子代就是带有某种基因的转基因鼠了。 问题2: dn-runx2应该是指dominant negetive-runx2,即是显性负作用突变体的runx2,一般都是人工改造的,顾名思意,这种负作用体通常带着某种缺失如酶功能的缺失等等,是没有功能的,甚至是起负作用的(如它能够结合到本来runx2应该起作用的位置,然后阻碍正常runx2的结合,从而抑制这个基因的作用
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