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- EASYBIO
- BE7012-100
- 2026年01月05日
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100ul/支
GST-HRP Mouse Monoclonal Antibody
GST tag Mouse mAb is the excellent antibody in the research. GST tag antibody can be helpful in detecting the fusion protein during purification as well as the cleavage of GST from the protein of interest. GST tag antibody has wide applications that could include your research on GST proteins or GST fusion recombinant proteins. GST Tag antibody can recognize C-terminal, internal, and N-terminal GST-tagged proteins.
Product Name: GST-tag mouse monoclonal antibody-HRP conjugated
Clone Number: 7D3
Isotype: IgG1
Immunogen: Full-length GST protein
Tested applications: WB
Recommended dilutions: WB: 1:2,000-5,000
Optimal dilutions/concentrations should be determined by the end user.
Form: Liquid
Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Storage: GST antibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Western blot analysis of GST-tag fusion protein with GST-tag mouse mAb(7D3) diluted at 1:5000.
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文献和实验Conjugation of Antibody to HRP
of Maleimide:HRP Weigh out 1.067 mg HRP for each mg of thiolated antibody. Add 1.1 mg Sulfo-SMCC per mg of HRP Add PBS to a final concentration of 8 mg/ml HRP Mix for 2 hours on a rotator. Desalt the HRP on PBS equilibrated column. Collect
Monoclonal Antibody Production Protocol
complete with MCM instead of PCM (= IMDM - m ), and keep it in the incubator for fused cells. Isolation of spleen cells Sacrifice mouse by cervical dislocation. Immerse mouse in 70% Ethanol. Remove spleen ( on the left side ) and transfer into a small
Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
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