- ¥200
- EASYBIO
- BE2032-10
- 2025年12月25日
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- 技术资料
- 形态：
液体
- 保存条件：
-20℃
- 克隆性：
多克隆
- 抗体英文名：
V5-Tag Mouse Monoclonal Antibody
- 规格：
10μl/支
V5 tag Mouse mAb is the excellent antibody in the research. V5 tag antibody can be helpful in detecting the recombinant proteins, some of which include transmembrane and secreted proteins fusion protein. V5 Tag Antibody can recognize C-terminal, internal, and N-terminal V5-tagged proteins.
Product Name:V5-tag mouse monoclonal antibody
Clone Number: 4C9
Isotype: IgG1
Immunogen: A synthetic peptide GKPIPNPLLGLDSTcoupled to KLH.
Tested applications and dilutions:WB 1:2000-5000 IP 1:200 IF 1:1000
Produce anti- V5 antibody affinity column purify V5 recombinant proteins
Form: Liquid
Concentration: 1mg/ml in PBS, pH 7.4, containing 0.02% sodium azide and 50% glycerol
Storage: V5antibody is stable for 1 month at 2-8°C. For long term storage, Aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
A: HEK 293 cell lysate transfected without V5-tag protein
B: HEK 293 cell lysate transfected with V5 -tag protein
C: IP (HEK 293 cell without transfected + anti- V5 mAb 4℃overnight)
D: IP (PBS + anti- V5 mAb 4℃overnight)
E: IP (HEK293 transfected+protein G+anti- V5 mAb 4℃overnight)
F: IP (HEK293 transfected+RIPA+Protein G 4℃overnight)
G: Multi Tag Recombinant protein (expressed in E.coli)
TP=Target Protein HC=Heavy chain LC=Light Chain
IF analysis of 293T cells transfected with a V5-tagged protein, using EASYBIO V5-Tag (4C9) Mouse mAb at a 1:2000 dilution (blue DAPI ,red anti-V5)
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文献和实验Monoclonal Antibody Production
and screening of the hybridomas: 1. Bleeding Mice (1) Place the mouse in a mouse restrainer. (2) Sterilize the tail with 70% ethanol. (3) With a razor blade,nip off the last 2 mm of the tip of the tail. (4) Using a milking motion,pull blood down and let
指仅对一种抗原决定基的纯粹的抗体。这类抗体不可能通过将抗原 胞的 Hybridoma，在试管内制成了纯粹的单克隆抗体。用某种抗原使动物产生免疫感应，对应于抗原之不同部分可制成各种不同的抗体复合体。然而，因为一个产生抗体的细胞，只能产生一种抗体，所以骨髓肿瘤细胞与产生抗体细胞间的细胞杂种，可形成边产生抗体边增殖的 hybridoma，将每个杂种细胞进行克隆（无性繁殖系）培养，则一个克隆的细胞群只由单种类产生同一抗体的细胞所组成，这样得到的就是单克隆抗体。此抗体在测定
monoclonal antibody production fusion
Fusion is an important procedure to produce monoclonal antibodies (MoAb). PROTOCOL prepare the culture of myeloma cells and maintain the concentration of the cells at the level of less than 10
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