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- 详细信息
- 文献和实验
- 技术资料
- 库存:
487
- 英文名:
Ribonuclease H
- 保质期:
长期
- 供应商:
北京百奥莱博科技有限公司
- 保存条件:
-20℃
特别声明:本产品及我公司所售其他产品均为科研类试剂产品,严禁用于药物、医疗及其他非科研用途。
北京百奥莱博专业生产供应RNase H厂商,我公司销*(代"售")全品类的分子生物学试剂,价格优惠,欢迎广大科研工作者垂询订购。
名称:RNase H厂商
编号:JN0012
品牌:百奥莱博
英文名:Ribonuclease H
规格:200U
RNase H是特异性分解RNA-DNA杂交体中的RNA链的核糖核酸内切酶,分子量为19,700,最适pH约为8.0。活性因子为Mg2+或Mn2+。活性单位为5U/ul。
产品组成:
| 组份 | JN0012-200U |
| RNase H (5U/ul) | 200ul |
| 10xRNase H Buffer | 1ml |
产品用途:
1、合成第二链cDNA时除去与第一链cDNA杂合的mRNA
2、DNA-RNA杂交体的鉴定
3、在Oligo(dT)存在下除去mRNA的Poly(A)末端
质量保证:经多次柱纯化,SDS-PAGE胶检测仅可见 清晰单一的目的条带;PCR方法检测无大肠杆菌DNA残留,无核酸内、外切酶污染。
活性定义:以poly(rA)•poly(dT)为底物,在30℃、pH7.7的条件下,20分钟内产生1 nmol酸可溶性物质所需要的酶量定义为1个活性单位(U)。
储存条件:-20℃
关于RNase H厂商的更详细说明,请致电我公司咨询,我们将以最饱满的热情为您提供解答,此外,我公司专业供应下列产品:
·重组人MICA
编号:JN1015
英文名称:Recombinant Human MHC Class I Polypeptide-Related Sequence A
规格:10μg|50μg|500μg|1mg
本品由我们的哺乳动物细胞表达系统制备而成,目的基因编码的Ala23-Glu308在C端带有Fc标签。
MICA质量控制:>95%(还原性SDS-PAGE)
MICA制剂:冻干品
MICA保存:
冻干蛋白置于-20℃以下可长期保存,室温条件下可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天,可分装后置于-20℃保存三个月。
MICA复溶:
打开试剂管前请先离心。
复溶浓度推荐大于100 μg/ml。
冻干蛋白请溶于ddH2O。
复溶后,请根据用量分装冻存,避免反复冻融。
关于MICA:
MHC class I polypeptide-related sequence A, also known as MIC-A, PERB11.1 and MICA, is a single-pass type I membrane protein which belongs to the MHC class I family of MIC subfamily. MICA contains one Ig-like C1-type domain and is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. MICA is the ligand for NK cell activating receptor KLRK1/NKG2D. MICA seems to have no role in antigen presentation. MICA leads to cell lysis by binding to KLRK1.
RNase H厂商关键词:JN0012,Ribonuclease H,RNase H
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载脂蛋白B(ApoB)检测试剂盒(免疫比浊比色法) 100T
ARB10318 人中性粒细胞趋化蛋白2(NAP-2/CXCL7)酶联免疫定量检测 Human neutrophil activating protein-2,nap-2 ELISA KIT
CBZ-甘*酸 5′-CTP,3Na 1138-80-3
BTN130531 组织培养专用蛋白酶抑制剂 Protease Inhibitor for Tissue Culture
PY08-036 乳糖发酵管(带导管) 10ml 10支/盒,用于大肠菌群的确证试验
沙黄/藏红T水溶液(2%) 100ml
ARB13280 小鼠颗粒酶B(Gzms-B)酶标法分析 Mouse granzymes b,gzms-b ELISA KIT
ARB10471 人白介素10(IL-10)含量测试 Human interleukin 10,IL-10 ELISA KIT
DAPI染色液(10ug/ml) 10ml|50ml
BTN3670 动物DNAOUT Animal DNAOUT
BL1337 dNTPs Mix(25mM each)
ARB13816 豚鼠载体蛋白B(Apo-B)定量分析
RNase H厂商关键词:JN0012,Ribonuclease H,RNase H
·重组人NLGN4X(神经连接蛋白4X)
编号:JN0836
英文名称:Recombinant Human Neuroligin 4, X-Linked
规格:10μg|50μg|500μg|1mg
本品由我们的哺乳动物细胞表达系统制备而成,目的基因编码的Gln42-Ser676在C端含有His标签。
NLGN4X质量控制:>95%(还原性SDS-PAGE)
NLGN4X制剂:冻干品
NLGN4X保存:
冻干蛋白置于-20℃以下可长期保存,室温条件下可稳定保存3周。
复溶蛋白溶液可在4~7℃保存2~7天,可分装后置于-20℃保存三个月。
NLGN4X复溶:
打开试剂管前请先离心。
复溶浓度推荐大于100 μg/ml。
冻干蛋白请溶于ddH2O。
复溶后,请根据用量分装冻存,避免反复冻融。
关于NLGN4X:
Neuroligin 4, X-Linked (NLGN4X) is a single-pass type I membrane protein that belongs to the type-B carboxylesterase/lipase family. NLGN4X is detected at higher levels in heart and at lower levels in the liver, skeletal muscle, and pancreas. NLGN4X is a putative neuronal cell surface protein involved in cell-cell-interactions. NLGN4X may act as splice site-specific ligands for β-neurexins. It has been shown that NLGN4X is involved in the formation and remodeling of central nervous system synapses. NLGN4X also interacts with discs, large (Drosophila) homolog 4 (DLG4). Defects in NLGN4X have been associated with autism and Asperger syndrome.
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文献和实验RNase H: Specificity, Mechanisms of Action, and Antiviral Target
The Ribonuclease (RNase) H is one of the four enzymes encoded by all retroviruses, including HIV. Its main activity is the hydrolysis of the RNA moiety in RNA–DNA hybrids. The RNase H ribonuclease is essential in the retroviral life cycle
Selective RNase H Cleavage of Target RNAs from a tRNA Scaffold
scaffold, using RNase H and two DNA oligonucleotides. After cleavage, we show that the RNA of interest can be isolated in a one-step purification. This method has, in particular, applications in structural investigations of RNA.
Question 1.What are the differences among RNase H, RNase A, RNase B and RNase C? 2.In your cDNA kits, RNase H is added in the second strand reaction to produce more nicked RNA as primers for DNA synthesis. In this repect
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