FITC-Labeled Recombinant Protein L 1200元/200ug, 6000元/2mg
分子别名(Synonym)
RPL,Protein L
表达区间及表达系统(Source)
FITC-Labeled Recombinant Protein L (RPL-PF141) is expressed from human HEK293 cells. It is the FITC labeled form of Recombinant Protein L (Cat # RPL-P3141).
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蛋白结构(Molecular Characterization)
FITC-Labeled Recombinant Protein L is fused with a polyhistidine tag at the N-terminus. The reducing (R) protein migrates as 42-47 kDa in SDS-PAGE.
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with FITC using standard chemical labeling method. The residual FITC is removed by molecular seive treatment during purification process.
生物素-蛋白标记度(FITC:Protein Ratio)
The FITC to protein molar ratio is 5-7 as determined by
内毒素(Endotoxin)
Less than 1.0 EU per μg by the LAL method.
纯度(Purity)
>95% as determined by SDS-PAGE.
制剂(Formulation)
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
重构方法(Reconstitution)
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
存储(Storage)
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
No activity loss is observed after storage at:
4-8°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
电泳(SDS-PAGE)
FITC-Labeled Recombinant Protein L on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
活性(Bioactivity)
Immobilized Yervoy Ipilumimab at 10 μg/mL (100 μL/well) can bind FITC-Labeled Recombinant Protein L (Cat. No. RPL-PF141) with a linear range of 20-156 ng/mL (QC tested).