- $495
- innov-research
- IRTPAKT
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- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50
- 英文名：
Rat tPA ELISA Kit
- 保质期：
十二个月
- 供应商：
信裕生物
- 保存条件：
-20°C
- 规格：
96 tests
Rat tPA ELISA Kit
Catalog No.: IRTPAKT
$495
Category: Assay and ELISA Kits
Product Description
Rat tPA ELISA Kit
Rat tPA ELISA Kit is for the sensitive quantitative measurement of functionally active rat tPA in plasma and other biological fluid samples is easily performed with this 96 well strip format ELISA kit. The level of active tPA in normal rat plasma was found to be 1.6 ng/ml. The assay measures active tPA in the 0.1-50 ng/ml range. Samples giving rat tPA levels above 50 ng/ml should be diluted in blocking buffer before use. Samples of plasma in citrate or EDTA may be assayed with this kit. Plasma in heparin is not recommended. It is important to ensure a platelet free preparation of plasma as platelets can release PAI-1, which in turn could potentially form a complex with active tPA. If plasma samples were collected in citrate the pH should be brought up to neutral with the 10X TBS provided in the kit. Serum and cell culture media at neutral pH may also be used.
Functionally active tPA will form a covalent complex with the biotinylated human PAI-1 which is bound to the avidin on the plate. Complexed tPA will not bind to the PAI-1 and will not be detected by the assay. After appropriate washing steps, anti-rat tPA primary antibody binds to the captured enzyme. Excess antibody is washed away, and bound antibody is reacted with the secondary antibody conjugated to HRP. Following an additional washing step, TMB substrate is used for color development at 450 nm. Color development is proportional to the concentration of active tPA in the samples. A standard calibration curve is prepared using dilutions of purified tPA and is measured along with the test samples. All reagents and standards are provided in these ELISA kits.
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
saline (PBS) (Cat. no. 10010-049) or equivalent Optional: ArC™ Amine Reactive Compensation Bead Kit (Cat. no. A10346) 实验步骤 1. Using the AbC™ anti- Rat/Hamster Bead Kit 1) Completely resuspend the AbC™ RH
RNA was purified using the AllPrep DNA/RNA 96 Kit and analyzed on the Agilent 2100 bioanalyzer. Intact RNA is indicated by a RIN value of close to 10. RNA with no genomic DNA contamination. Total RNA was purified from rat kidney
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