APC标记小鼠抗人CD8单克隆抗体

英文名称human CD8-APC
中文名称APC标记小鼠抗人CD8单克隆抗体
别    名CD8-b;CD 8A; CD 8B; CD8 antigen alpha polypeptide; CD8A anti; CD8a antigen an; CD8a molecule; CD8B; CD8b antigen; CD8b antigen isoform 2; CD8b molecule; CD8B protein; CD8B1; Leu2; Leu2 T lymphocyte antigen; Ly3; LYT3; MAL; MGC119115; OKT8 T Cell antigen; p32; T cell antigen Leu2; T cell surface glycoprotein CD8 alpha chain; T cell surface glycoprotein CD8 beta chain; T lymphocyte differentiation antigen T8/Leu 2; CD8A_HUMAN.  APC anti-human CD8;
规格25T  100T  
研究领域细胞生物  免疫学  干细胞  细胞表面分子  淋巴细胞  t-淋巴细胞  b-淋巴细胞  
抗体来源Mouse
克隆类型Monoclonal
交叉反应 Human, 
产品应用WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=20ul/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量27kDa
细胞定位细胞膜 分泌型蛋白 
性    状Liquid
免 疫 原: 
亚    型IgG
纯化方法affinity purified by Protein G
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMedPubMed
产品介绍background:
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011].

Subunit:
In general heterodimer of an alpha and a beta chain linked by two disulfide bonds. Can also form homodimers. Shown to be expressed as heterodimer on thymocytes and as homodimer on peripheral blood T-lymphocytes. Interacts with the MHC class I HLA-A/B2M dimer. Interacts with LCK in a zinc-dependent manner.

Subcellular Location:
Isoform 1: Cell membrane; Single-pass type I membrane protein. 
Isoform 2: Secreted.

Similarity:
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

SWISS:
P01732

Gene ID:
925

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

产品图片	Flow cytometry staining of normal human peripheral blood cells with CD8/APC (bsm-30069M-APC)(Red histogram) or Mouse IgG1 Isotype Control (APC Conjugate)(C03-11004 )(Blue histogram) . Total cells were used for analysis.