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- 2025年11月11日
- WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
- 详见说明书
- 详见说明书
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- 详细信息
- 技术资料
- 抗体名：
磷酸化复制因子A蛋白2抗体
- 抗体英文名：
Anti-phospho-RPA32/RPA2(Thr21)
- 靶点：
详见说明书
- 浓度：
1mg/1ml
- 应用范围：
WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
- 宿主：
详见说明书
- 供应商：
上海一研
- 库存：
41
- 级别：
详见说明书
- 目录编号：
详见说明书
- 抗原来源：
Rabbit
- 保质期：
详见说明书
- 适应物种：
详见说明书
- 标记物：
详见说明书
- 克隆性：
多克隆
- 保存条件：
Store at -20 °C
- 形态：
详见说明书
- 亚型：
IgG
- 免疫原：
KLH conjugated Synthesised phosphopeptide derived from human RPA2 around the phosphorylation site of Thr21
- 规格：
0.1ml/100μg
英文名称 Anti-phospho-RPA32/RPA2(Thr21)
中文名称 磷酸化复制因子A蛋白2抗体价格
别 名 RPA32/RPA2(phospho T21); 60S acidic ribosomal protein P1; p32; p34; REPA2; Replication factor A protein 2; Replication protein A 32 kDa subunit; Replication protein A 32kDa subunit; Replication protein A 34 kDa subunit; Replication protein A; Replication Protein A2 (32kDa); Replication protein A2; Replication protein A2, 32kDa; RF-A protein 2; Rf-A2; RFA; RFA2_HUMAN; RP-A p32; RP-A p34; RP21C; RPA 2; RPA 32; RPA; Rpa2; RPA32; RPA34; RpLP1; RpP2.
浓 度 1mg/1ml
规 格 0.1ml/100μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow
产品类型 一抗 磷酸化抗体
研究领域 免疫学 染色质和核信号
蛋白分子量 predicted molecular weight: 29 kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human RPA2 around the phosphorylation site of Thr21
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Function : Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Subunit : Heterotrimer of 70, 32 and 14 kDa chains (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN. Directly interacts with PPP4R2, but not with SMEK2; this interaction is DNA damage-dependent and leads RPA2 dephosphorylation by PPP4C recruitment. Interacts with RAD51, preferentially when hyperphosphorylated. Directly interacts with RFWD3.
Subcellular Location : Nucleus. Nucleus, PML body. Note=Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
Post-translational modifications : Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). In response to DNA damage, recruited to DNA-repair nuclear foci, as a hypophosphorylated form. The necessary dephosphorylation step is catalyzed by PP4. Subsequent hyperphosphorylation, catalyzed by ATR, is required for RAD51 recruitment to chromatin and efficient DNA repair. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1. Phosphorylation at Thr-21 depends upon RFWD3 presence.
Database links : UniProtKB/Swiss-Prot: P
磷酸化复制因子A蛋白2抗体价格的功能
抗体的主要功能是与抗原（包括外来的和自身的）相结合，从而有效地清除侵入机体内的微生物、寄生虫等异物，抗体（antibody）是一种应答抗原产生的、可与抗原特异性结合的蛋白质。每种抗体与特定的抗原决定基结合。这种结合可以使抗原失活，也可能无效但有时也会对机体造成病理性损害，如抗核抗体、抗双链DNA抗体、抗甲状腺球蛋白抗体等一些自身抗体的产生，对人体可造成危害。
公司抗体应用于WB、IHC、IF、ELISA等实验中，磷酸化复制因子A蛋白2抗体价格品质保证，提供完善的售前、售中、售后服务！欢迎来电咨询或联系在线客服！我们将竭诚为您服务！
磷酸化复制因子A蛋白2抗体价格的优点：
①耐热、耐酸、耐碱、抗有机溶剂以及金属离子，稳定性好，室温下可以长期保存；
②制备简单，操作方便，可以进行批量；
③不必免疫动物，且可获得免疫动物所不能得到的“抗体”；
④可以反复使用；
⑤价格低廉。因此，设计、合成既具有类似生物抗体的高亲合性和高专一性，又具有耐热、耐酸、耐碱且又可以长期稳定的人工抗体，在部分领域替代生物抗体以进行仿生分子识别，或者完成一些生物抗体所不能完成的工作，具有重要的科学意义，在化学、生命科学和环境科学等方面具有广阔的应用前景
公司专业供应各种进口、国产一抗及二抗，品牌有R&D、Santa Cruz、Bipec、Millipore等国际知名品牌，品种多达7000多种，由于产品的数量众多，产品的宿主、抗原来源及应用范围(WB、IH(F/P)、FC、ELISA、IP、IF等)有所不同。
磷酸化复制因子A蛋白2抗体价格具有高度的特异性，免疫组织化学正是利用了这一原理。先将组织或细胞中的某种化学物质提取出来，以此作为抗原或半抗原，通过免疫动物后获得特异性的抗体，再以此抗体去探测组织或细胞中的同类的抗原物质。由于抗原与抗体的复合物是无色的，因此还必须借助于组织化学的方法将抗原抗体结合的部位显示出来，以其达到对组织或细胞中的未知抗原进行定性，定位或定量的研究。
磷酸化复制因子A蛋白2抗体价格包括以下几个步骤：
1、制备抗原。
2、选择实验动物。
3、动物免疫。
4、试取血进行测试，看看是否成功免疫。
5、如果成功免疫，杀死实验动物，采集全部血清。
6、纯化出抗体。
7、鉴定抗体。包括纯度以及特异性。
phospho-NFATc2(Ser326)磷酸化核因子活化T细胞胞浆蛋白2抗体规格:0.1ml
Borealin/CDCA8细胞分裂周期蛋白8抗体规格:0.2ml
phospho-c-Raf(Tyr341)磷酸化原基因c-Raf抗体规格:0.1ml
CK19/CK17/CK14/CK10/CK42细胞角蛋白19，17，14，42，10抗体规格:0.1ml
HE4/EpididymalsecretoryproteinE4附睾分泌蛋白4抗体0.2ml
APBA3/Mint3/X11gammaβ淀粉样前体蛋白结合蛋白3抗体规格:0.2ml
RPL19核糖体蛋白L19抗体规格:0.2ml
phospho-MEF2D(Ser180)磷酸化肌细胞特异性增强因子2D抗体规格:0.1ml
PAP-α/β/γ多聚合酶α/β/γ抗体规格:0.2ml
MouseAnti-BovIgG/AlexaFluor488AlexaFluor488标记的小鼠抗牛IgG规格:0.1ml
ANKLE2ANKLE2蛋白抗体规格:0.2ml
GDF8生分化因子8/肌肉抑制素抗体0.2mlPRRS-RSAB-N Ala-Gly-Gly-Ser-Ser-Ser-Thr-Tyr-Gln-Tyr
Scyliorhinin I, Scy I； Shark Substance P Related Peptide Ala-Lys-Phe-Asp-Lys-Phe-Tyr-Gly-Leu-Met
Substance P (1-4) Arg-Pro-Lys-Pro
Type B Allatostatin Ala-Tyr-Ser-Tyr-Val-Ser-Glu-Tyr-Lys-Arg-Leu-Pro-Val-Tyr-Asn-Phe-Gly-Leu-NH2
W-K-Y-M-V-M-NH2 Trp-Lys-Tyr-Met-Val-Met-NH2
β- Amyloid (37- 43) Gly-Gly-Val-Val-Ile-Ala-Thr
β-Defensin-3, human Gly-Ile-Ile-Asn-Thr-Leu-Gln-Lys-Tyr-Tyr-Cys-Arg-Val-Arg-Gly-Gly-Arg-Cys-Ala-Val-Leu-Ser-Cys-Leu-Pro-Lys-Glu-Glu-Gln-Ile-Gly-Lys-Cys-Ser-Thr-Arg-Gly-Arg-Lys-Cys-Cys-Arg-Arg-Lys-Lys (Disulfide bridge: Cys11-Cys40, Cys18-Cys33, Cys23-Cys41)
磷酸化复制因子A蛋白2抗体价格[Asp371] Tyrosinase(369-377), human Tyr-Met-Asp-Gly-Thr-Met-Ser-Gln-Val
[Des-Gly10,Dleu6, ProNHEt9] LH-RH, AcetateLeuprolide, Acetate Pyr-His-Trp-Ser-Tyr-D-Leu-Leu-Arg-Pro-NHEt
[D-Val22] Big Endothelin-1 (16-38), human His-Leu-Asp-Ile-Ile-Trp-D-val-Asn-Thr-Pro-Glu-His-Val-Val-Pro-Tyr-Gly-Leu-Gly-Ser-Pro-Arg-Ser
[Met5,Arg6,7,Val8,Gly9] Enkephalin Tyr-Gly-Gly-Phe-Met-Arg-Arg-Val-Gly
[Ser25]-PKC (19-36) Substrate Arg-Phe-Ala-Arg-Lys-Gly-Ser-Leu-Arg-Gln-Lys-Asn-Val-His-Glu-Val-Lys-Asn
[Tyr8,Nle11] Substance P Arg-Pro-Lys-Pro-Gln-Gln-Phe-Tyr-Gly-Leu-Nle-NH2
Ac-Amyloid β-Protein (15-20) amide Ac-Gln-Lys-Leu-Val-Phe-Phe- NH2
Ac-β-Endorphin (human) Ac-Tyr-Gly-Gly-Phe-Met-Thr-Ser-Glu-Lys-Ser-Gln-Thr-Pro-Leu-Val-Thr-Leu-Phe-Lys-Asn-Ala-Ile-Ile-Lys-Asn-Ala-Tyr-Lys-Lys-Gly-Glu
中文名称 磷酸化复制因子A蛋白2抗体价格
别 名 RPA32/RPA2(phospho T21); 60S acidic ribosomal protein P1; p32; p34; REPA2; Replication factor A protein 2; Replication protein A 32 kDa subunit; Replication protein A 32kDa subunit; Replication protein A 34 kDa subunit; Replication protein A; Replication Protein A2 (32kDa); Replication protein A2; Replication protein A2, 32kDa; RF-A protein 2; Rf-A2; RFA; RFA2_HUMAN; RP-A p32; RP-A p34; RP21C; RPA 2; RPA 32; RPA; Rpa2; RPA32; RPA34; RpLP1; RpP2.
浓 度 1mg/1ml
规 格 0.1ml/100μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow
产品类型 一抗 磷酸化抗体
研究领域 免疫学 染色质和核信号
蛋白分子量 predicted molecular weight: 29 kDa
性 状 Lyophilized or Liquid
免 疫 原 KLH conjugated Synthesised phosphopeptide derived from human RPA2 around the phosphorylation site of Thr21
亚 型 IgG
纯化方法 affinity purified by Protein A
储 存 液 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide
产品应用 WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Function : Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions. Required for the efficient recruitment of the DNA double-strand break repair factor RAD51 to chromatin in response to DNA damage.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
Subunit : Heterotrimer of 70, 32 and 14 kDa chains (canonical replication protein A complex). Component of the alternative replication protein A complex (aRPA) composed of RPA1, RPA3 and RPA4. The DNA-binding activity may reside exclusively on the 70 kDa subunit. Binds to SERTAD3/RBT1. Interacts with TIPIN. Directly interacts with PPP4R2, but not with SMEK2; this interaction is DNA damage-dependent and leads RPA2 dephosphorylation by PPP4C recruitment. Interacts with RAD51, preferentially when hyperphosphorylated. Directly interacts with RFWD3.
Subcellular Location : Nucleus. Nucleus, PML body. Note=Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
Post-translational modifications : Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). In response to DNA damage, recruited to DNA-repair nuclear foci, as a hypophosphorylated form. The necessary dephosphorylation step is catalyzed by PP4. Subsequent hyperphosphorylation, catalyzed by ATR, is required for RAD51 recruitment to chromatin and efficient DNA repair. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1. Phosphorylation at Thr-21 depends upon RFWD3 presence.
Database links : UniProtKB/Swiss-Prot: P
磷酸化复制因子A蛋白2抗体价格的功能
抗体的主要功能是与抗原（包括外来的和自身的）相结合，从而有效地清除侵入机体内的微生物、寄生虫等异物，抗体（antibody）是一种应答抗原产生的、可与抗原特异性结合的蛋白质。每种抗体与特定的抗原决定基结合。这种结合可以使抗原失活，也可能无效但有时也会对机体造成病理性损害，如抗核抗体、抗双链DNA抗体、抗甲状腺球蛋白抗体等一些自身抗体的产生，对人体可造成危害。
公司抗体应用于WB、IHC、IF、ELISA等实验中，磷酸化复制因子A蛋白2抗体价格品质保证，提供完善的售前、售中、售后服务！欢迎来电咨询或联系在线客服！我们将竭诚为您服务！
磷酸化复制因子A蛋白2抗体价格的优点：
①耐热、耐酸、耐碱、抗有机溶剂以及金属离子，稳定性好，室温下可以长期保存；
②制备简单，操作方便，可以进行批量；
③不必免疫动物，且可获得免疫动物所不能得到的“抗体”；
④可以反复使用；
⑤价格低廉。因此，设计、合成既具有类似生物抗体的高亲合性和高专一性，又具有耐热、耐酸、耐碱且又可以长期稳定的人工抗体，在部分领域替代生物抗体以进行仿生分子识别，或者完成一些生物抗体所不能完成的工作，具有重要的科学意义，在化学、生命科学和环境科学等方面具有广阔的应用前景
公司专业供应各种进口、国产一抗及二抗，品牌有R&D、Santa Cruz、Bipec、Millipore等国际知名品牌，品种多达7000多种，由于产品的数量众多，产品的宿主、抗原来源及应用范围(WB、IH(F/P)、FC、ELISA、IP、IF等)有所不同。
磷酸化复制因子A蛋白2抗体价格具有高度的特异性，免疫组织化学正是利用了这一原理。先将组织或细胞中的某种化学物质提取出来，以此作为抗原或半抗原，通过免疫动物后获得特异性的抗体，再以此抗体去探测组织或细胞中的同类的抗原物质。由于抗原与抗体的复合物是无色的，因此还必须借助于组织化学的方法将抗原抗体结合的部位显示出来，以其达到对组织或细胞中的未知抗原进行定性，定位或定量的研究。
磷酸化复制因子A蛋白2抗体价格包括以下几个步骤：
1、制备抗原。
2、选择实验动物。
3、动物免疫。
4、试取血进行测试，看看是否成功免疫。
5、如果成功免疫，杀死实验动物，采集全部血清。
6、纯化出抗体。
7、鉴定抗体。包括纯度以及特异性。
phospho-NFATc2(Ser326)磷酸化核因子活化T细胞胞浆蛋白2抗体规格:0.1ml
Borealin/CDCA8细胞分裂周期蛋白8抗体规格:0.2ml
phospho-c-Raf(Tyr341)磷酸化原基因c-Raf抗体规格:0.1ml
CK19/CK17/CK14/CK10/CK42细胞角蛋白19，17，14，42，10抗体规格:0.1ml
HE4/EpididymalsecretoryproteinE4附睾分泌蛋白4抗体0.2ml
APBA3/Mint3/X11gammaβ淀粉样前体蛋白结合蛋白3抗体规格:0.2ml
RPL19核糖体蛋白L19抗体规格:0.2ml
phospho-MEF2D(Ser180)磷酸化肌细胞特异性增强因子2D抗体规格:0.1ml
PAP-α/β/γ多聚合酶α/β/γ抗体规格:0.2ml
MouseAnti-BovIgG/AlexaFluor488AlexaFluor488标记的小鼠抗牛IgG规格:0.1ml
ANKLE2ANKLE2蛋白抗体规格:0.2ml
GDF8生分化因子8/肌肉抑制素抗体0.2mlPRRS-RSAB-N Ala-Gly-Gly-Ser-Ser-Ser-Thr-Tyr-Gln-Tyr
Scyliorhinin I, Scy I； Shark Substance P Related Peptide Ala-Lys-Phe-Asp-Lys-Phe-Tyr-Gly-Leu-Met
Substance P (1-4) Arg-Pro-Lys-Pro
Type B Allatostatin Ala-Tyr-Ser-Tyr-Val-Ser-Glu-Tyr-Lys-Arg-Leu-Pro-Val-Tyr-Asn-Phe-Gly-Leu-NH2
W-K-Y-M-V-M-NH2 Trp-Lys-Tyr-Met-Val-Met-NH2
β- Amyloid (37- 43) Gly-Gly-Val-Val-Ile-Ala-Thr
β-Defensin-3, human Gly-Ile-Ile-Asn-Thr-Leu-Gln-Lys-Tyr-Tyr-Cys-Arg-Val-Arg-Gly-Gly-Arg-Cys-Ala-Val-Leu-Ser-Cys-Leu-Pro-Lys-Glu-Glu-Gln-Ile-Gly-Lys-Cys-Ser-Thr-Arg-Gly-Arg-Lys-Cys-Cys-Arg-Arg-Lys-Lys (Disulfide bridge: Cys11-Cys40, Cys18-Cys33, Cys23-Cys41)
磷酸化复制因子A蛋白2抗体价格[Asp371] Tyrosinase(369-377), human Tyr-Met-Asp-Gly-Thr-Met-Ser-Gln-Val
[Des-Gly10,Dleu6, ProNHEt9] LH-RH, AcetateLeuprolide, Acetate Pyr-His-Trp-Ser-Tyr-D-Leu-Leu-Arg-Pro-NHEt
[D-Val22] Big Endothelin-1 (16-38), human His-Leu-Asp-Ile-Ile-Trp-D-val-Asn-Thr-Pro-Glu-His-Val-Val-Pro-Tyr-Gly-Leu-Gly-Ser-Pro-Arg-Ser
[Met5,Arg6,7,Val8,Gly9] Enkephalin Tyr-Gly-Gly-Phe-Met-Arg-Arg-Val-Gly
[Ser25]-PKC (19-36) Substrate Arg-Phe-Ala-Arg-Lys-Gly-Ser-Leu-Arg-Gln-Lys-Asn-Val-His-Glu-Val-Lys-Asn
[Tyr8,Nle11] Substance P Arg-Pro-Lys-Pro-Gln-Gln-Phe-Tyr-Gly-Leu-Nle-NH2
Ac-Amyloid β-Protein (15-20) amide Ac-Gln-Lys-Leu-Val-Phe-Phe- NH2
Ac-β-Endorphin (human) Ac-Tyr-Gly-Gly-Phe-Met-Thr-Ser-Glu-Lys-Ser-Gln-Thr-Pro-Leu-Val-Thr-Leu-Phe-Lys-Asn-Ala-Ile-Ile-Lys-Asn-Ala-Tyr-Lys-Lys-Gly-Glu
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磷酸化复制因子A蛋白2抗体价格
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