斑马鱼TGF-β1/2抗体抗原介绍 Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Heterodimers of TGFB1/TGFB2 have been found in bone. Interacts with CD109 and DPT. Interacts with ASPN. Function: TGF beta 2 has suppressive effects on interleukin-2 dependent T-cell growth. Tissue specificity: TGF beta 1 is highly expressed in bone. A chromosomal aberration involving TGFB2 is found in a family with Peters anomaly.
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斑马鱼TGF-β1/2抗体Recommended Dilutions:WB: 1:500-1:1,000 ICC: 1:200 IHC: 1:200 FC:1:100
Fig1: Western blot analysis of TGF-Beta 1/2 on differentcell
lysates using anti-TGF-Beta 1/2 antibody at 1/1000 dilution. Positive control: Lane 1: Raji Lane2: MCF-2Lane 3: A549 Lane 4: Human kidney
Fig2: ICC staining TGF-Beta 1/2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining TGF-Beta 1/2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining TGF-Beta 1/2 in SKBR-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TGF-Beta 1/2 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TGF-Beta 1/2 antibody. Counter stained with hematoxylin.
Fig6:Flow cytometric analysis of HepG2 cells with TGF-Beta 1/2 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.斑马鱼TGF-β1/2抗体