- ¥2500
- 上海雅吉生物科技有限公司
- 40113
- 中国
- 2025年08月21日
- ICC , IHC , WB
- 兔多抗
- Mouse , Human , Zebrafish , Hybrid fish
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- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海雅吉生物科技有限公司
- 库存：
150
- 靶点：
Cytoplasm
- 克隆性：
多克隆
- 抗原来源：
兔多抗
- 保质期：
一年
- 抗体英文名：
Desmin
- 抗体名：
Desmin
- 宿主：
兔多抗
- 适应物种：
Mouse , Human , Zebrafish , Hybrid fish
- 亚型：
IgG
- 形态：
液体
- 应用范围：
ICC , IHC , WB
- 浓度：
1mg/ml
- 保存条件：
-20°
Human heart tissue, human skeletal muscle tissue, mouse heart tissue, human uterus tissue, mouse skeletal muscle tissue, HepG2,NIH-3T3,Hela, hybrid fish (crucian-carp) brain tissue lysate.
更多斑马鱼抗体详情，请来电咨询我们，我们将竭诚为您服务
斑马鱼Desmin抗体抗原介绍 ： Desmin is one of the earliest protein markers for muscle tissue in embryogenesis as it is detected in the somites. Although it is present early in the development of muscle cells, it is only expressed at low levels, and increases as the cell nears terminal differentiation. Desmin is also important in muscle cell architecture and structure since it connects many components of the cytoplasm. Finally, desmin may be important in mitochondria function. Desmin-related myopathy (DRM or Desminopathy) is a subgroup of the myofibrillar myopathy diseases and is the result of a mutation in the gene that codes for desmin which prevents it from forming protein filaments, instead forming aggregates of desmin and other proteins throughout the cell. Recently, mutations were identified in patients suffered by an arrhythmogenic right ventricular cardiomyopathy (ARVC).
Recommended Dilutions: WB: 1:1,000-1:2,000 ICC: 1:200 IHC: 1:200
.png)
Fig1: Western blot analysis of Desmin on
differentcell lysates using anti-Desmin
antibody at 1/2000 dilution. Positive control: Lane 1: Human skeletal muscle tissue Lane 2: Mouse heart tissue Lane 3: Human heart tissue，斑马鱼Desmin抗体
Fig2: ICC staining of Desminin HepG2 cells (green). Cells
were fixed in paraformaldehyde, permeabilised with 0.25%
Triton X100/PBS.
斑马鱼Desmin抗体，Fig3: ICC staining of Desmin in NIH-3T3 cells (green).
Cells were fixed in paraformaldehyde, permeabilised with
0.25% Triton X100/PBS.
Fig4: ICC staining of Desmin in Hela cells (green). Cells
were fixed in paraformaldehyde, permeabilised with 0.25%
Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded
human uterus tissue using anti-Desminantibody. Counter
stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded
mouse skeletal muscle tissue using anti-Desminantibody.
Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded
mouse heart tissue using anti-Desminantibody. Counter
stained with hematoxylin.
Fig8: Western blot analysis of Desmin on hybrid fish (crucian-carp) brain tissue lysate using anti-Desmin antibody
斑马鱼Desmin抗体
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人结蛋白 ( Desmin )ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人Desmin 单抗包被于酶标板上，标准品和样品中的 Desmin与单抗结合，加入生物素化的抗人 Desmin
排列。当细胞融合80%以上后，开始形成肌管。2、免疫细胞化学染色1：肌卫星细胞胞浆中含有结蛋白（desmin），可采用鼠抗人及小鼠desmin一抗对卫星细胞进行免疫染色鉴定。3、免疫细胞化学染色2：采用骨骼肌特异性的肌球蛋白（myosin）单克隆抗体检测。取含骨骼肌细胞盖玻片常规处理后进行myosin的细胞免疫化学染色，PBS代替一抗做阴性对照。结果判定：以细胞浆出现棕黄色颗粒为阳性染色。六、注意事项1、胰酶消化过程中，也可采用15min两步消化的方法进行。根据所取组织个体年龄决定，一般成年大小
0:00 斑马鱼全标本包埋的高分辨率双荧光原位杂交0 0:21 订阅内容21 1:00 内容简介60 1:27 固定胚胎、去卵膜87 3:10 胚胎透化处理190 3:51 杂交231 5:37 抗体标记337 9:10 碘化丙啶染色、检测550 10:31 结果/成果631 11:41 结论701 斑马鱼全标本包埋的高分辨率双荧光原位杂交本视频来源于网络，如有异议请联系我们，我们将在5个工作日内作出处理。
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