- $495
- innov-research
- IRKTAH1147
- 美国/英国
- 2025年07月13日
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- 文献和实验
- 技术资料
- 库存：
50
- 英文名：
Mouse Alpha-1 Antitrypsin ELISA Kit
- 保质期：
十二个月
- 供应商：
信裕生物
- 保存条件：
-20°C
- 规格：
96 tests
Mouse Alpha-1 Antitrypsin ELISA Kit
Catalog No.: IRKTAH1147
$495
Category: Assay and ELISA Kits
Product Description
Mouse Alpha-1 Antitrypsin ELISA Kit
In this assay the A1AT present in samples reacts with the anti-A1AT antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-A1AT antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound A1AT . Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3,5,5-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of A1AT in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of A1AT in the test sample. The quantity of A1AT in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
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文献和实验RAT/MOUSE GROWTH HORMONE ELISA KIT
实验原理 This assay is a Sandwich ELISA based, sequentially, on: 1) capture of rat or mouse Growth Hormone molecules from samples to the wells of a microtiter plate coated by a pre-titered amount of anti-Growth Hormone
每孔上样总蛋白量一致，实现样本间公平对比。 三、实战 protocol：肿瘤组织 IFN-γ 检测完整流程 以 “磁控 CAR-T 模拟细胞治疗后，肿瘤组织 IFN-γ 浓度检测” 为例，操作如下： 1.实验准备：试剂（abs9225、abs9161、abs9232、Mouse IFN-γ ELISA Kit 等）、仪器（组织处理器、高速冷冻离心机、酶标仪等）、治疗组与对照组小鼠肿瘤组织。 2. 组织裂解：肿瘤组织离体后用 PBS 冲洗吸干，精准称量 10mg 放入研磨管；加入 100μl 现配
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
Materials Lyophilized rat/mouse PINP calibrators 0 to 5 and controls 1 and 2 (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/): reconstitute calibrators
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