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CD34分子(CD34)ELI
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库存1226套
赛泓瑞生物
0.313-20ng/mL The standard curve concentrations used for the ELISA’s were 20ng/mL, 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL
酶联免疫吸附试验法
ELISA Kit for Cluster Of Differentiation 34 (CD34)
Homo sapiens (Human)
血清、血浆、尿液、细胞培养上清、组织标本
48T/96T
1.Add 100 μL of standard or sample to each well.
2.Remove the liquid.
3.Add 100 μL Biotinylated Detection Ab. Incubate 1 hour at 37℃.Aspirate and wash 3 times.
4.Add 100 μL HRP Conjugate. Incubate 30 min at 37℃.Aspirate and wash 5 times.
5.Add 90 μL of Substrate Reagent. Incubate for 15 min at 37℃.
6.Add 50 μL of Stop Solution.
7.Read the OD at 450 nm immediately. Calculation of the results.
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