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- 详细信息
- 文献和实验
- 技术资料
- 库存：
需确认
- 英文名：
Di(adenosine-5'-)penta-phosphate
- CAS号：
75522-97-3
- 保质期：
24个月
- 供应商：
嵘崴达
- 保存条件：
+2 to +8°C
Di(adenosine-5'-)penta-phosphate
trilithium salt 10 161 624 103
Application
Use Di(adenosine-5'-)penta-phosphate in diagnostic reagents for the determination
of creatine kinase to inhibit adenylate kinase in the reaction.
Benefits
Rely on the proven diagnostic quality of this product.
CAS: 75522-97-3
Properties
Formula: C20H26N10O22P5Li3
Molecular weight: 934.2 D (Ap5A: 916.4 D)
Specification
Appearance: White to slightly yellowish, amorphous powder
Solubility: Clear, colorless to slightly yellowish solution in water (c=10 mg/ml)
Ap5A-Li3 (from Porganic): ≥91%
Ap5A (A260, ε=26.4 [l x mmol-1 x cm-1]): ≥90%
Ap5A (from Porganic): ≥90%
Ap5A (HPLC): ≥95 area%
Li (flame photometric): 2.1±0.3%
Water (K. Fischer): ≤5%
Ptotal (ammonium vanadate): ≥15.2%
Pi (ammonium vanadate): ≤1.5%
Thin layer chromatography (PEI-cellulose, KH2PO4, 0.75 mol/l): Chromatographically
homogeneous
A250/A260: 0.79±0.04
A280/A260: 0.21±0.03
A290/A260: 0.02±0.02
Stability: At +2 to +8°C within specification range for 24 months.
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文献和实验4.Transfer clarified extract to 50 ml tube 5.During spin prepare Ni-Sepharose: wash with 5 volumes DI water,wash with 5 volumes Binding buffer,resuspend Ni-Sepharose to 50% in Binding buffer (spins are at 500 x g for 2'-5') 6.Add 2 ml 50% Ni-Sepharose
Crystallization of Kinesin Family Motor Proteins
(2-carboxyethyl)-phosphineReservoir: 20% polyethylene glycol 3350 in 0.2 M di-sodium hydrogen phosphate, pH 9.1Vinogradova et al., 2004KinISitting drop4°C10 - 20 mg/ml protein Reservoir: 1.4-1.8 M ammonium sulfate, 100 mM sodium acetate (pH 5.0), 200 mM sodium
of the propargylated c‐di‐GMP 9 from the propargylated dinucleoside phosphate triester 5 . Abbreviations: GiBu , 2‐( N ‐isobutyryl)guanin‐9‐yl; TBDMS, tert ‐butyldimethylsilyl; Gua, guanin‐9‐yl. Adapted with permission from Grajkowski et al. (). Copyright 2010 American
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