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- 文献和实验
- 技术资料
- 库存:
50
- 英文名:
Human LAP TGF-beta1 ELISA Kit
- 保质期:
十二个月
- 供应商:
信裕生物
- 保存条件:
-20°C
- 规格:
96 tests
Human LAP TGF-beta1 ELISA Kit
Catalog No.: IRAPKT4021
$495
Category: Assay and ELISA Kits
Product Description
Human LAP TGF-beta1 ELISA Kit
The Human LAP TGF-b1 ELISA kit is designed for detection of LAP TGF-b1 in plasma, serum, milk, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures LAP TGF-b1 in less than 5 hours. A murine monoclonal antibody specific for human LAP TGF-b1 has been pre-coated onto a microplate. LAP TGF-b1 in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for human LAP TGF-b1, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.Related Products
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文献和实验调至 1μg/μl。 4. ELISA 检测:按试剂盒说明书完成板平衡、加样孵育、洗板、酶标抗体孵育、显色终止与读数,结合标准曲线得出 IFN-γ 浓度。 四、操作要点速记 1.组织样本新鲜处理,全程低温操作,裂解液现配现用; 2. 蛋白定量时标准品与样本同步孵育,稀释采用倍比稀释减少误差; 3. ELISA 实验需每次重绘标准曲线,洗板充分、严格把控显色与终止时间。 免疫检测试剂盒: 货号-规格 名称 abs510001-96T Human IL-2 ELISA Kit (Faster
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