磷酸化上皮细胞癌转化蛋白2抗体品牌

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英文名称  Anti-phospho-ECT2 (Ser370) 
中文名称  磷酸化上皮细胞癌转化蛋白2抗体品牌   
别    名  ECT2 (phospho S370); ECT2 (phospho Ser370); p-ECT2 (Ser370); ECT 2; ECT2; ECT-2; ECT2 protein; Epithelial cell transforming sequence 2; Epithelial cell transforming sequence 2 oncogene; Epithelial cell transforming sequence 2 oncogene protein; FLJ10461; MGC138291; Protein ECT2; ECT2_HUMAN; Epithelial cell-transforming sequence 2 oncogene. 
浓    度  1mg/1ml 
规 格  0.1ml/200μg     
抗体来源  Rabbit  
克隆类型  polyclonal 
交叉反应  Human   
产品类型  一抗  磷酸化抗体   
研究领域  
性    状  Lyophilized or Liquid 
免 疫 原  KLH conjugated synthesised phosphopeptide derived from human ECT2 around the phosphorylation site of Ser370 
亚    型  IgG 
纯化方法  affinity purified by Protein A 
储 存 液  Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 
产品应用   WB=1:100-500  ELISA=1:500-1000  IP=1:20-100  IHC-P=1:100-500  IHC-F=1:100-500  ICC=1:100-500  IF=1:100-500 
（石蜡切片需做抗原修复） 
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.  
保存条件  Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
Important Note  This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
产品介绍 The protein encoded by this gene is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis. [provided by RefSeq].
Function : Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits Phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
Subunit : Interacts with NR1I3 (By similarity). Homodimer. Homooligomer. Found in the centralspindlin complex. Interacts (Thr-359 phosphorylated form) with PARD6A; the interaction is observed in cancer cells. Interacts (Thr-359 phosphorylated form) with PRKCI; the interaction is observed in cancer cells. Interacts with PKP4; the interaction is observed at the midbody. Interacts with RACGAP1; the interaction is direct, occurs in a microtubule-dependent manner, is inhibited in metaphase by phosphorylation of ECT2 on Thr-373 and is stimulated in early anaphase by dephosphorylation of ECT2 probably on Thr-373 through CDK1 activity. Interacts with PLK1; the interaction is stimulated upon its phosphorylation on Thr-444. Associates with RACGAP1 at anaphase and during cytokinesis. Interacts with KIF23, PARD3, PARD6A, PARD6B and PRKCQ.
Subcellular Location : Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cleavage furrow. Midbody. Cell junction. Cell junction, tight junction. Note=Sequestered within the nucleus during interphase. Dispersed throughout the cytoplasm upon breakdown of the nuclear envelope during mitosis. Colocalizes with the centralspindlin complex to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalized with RhoA at the midbody. Its subcellular localization to tight junction is increased by calcium. Localized predominantly in the cytoplasm of numerous carcinoma cells.
Tissue Specificity : Expressed in lung epithelial cells (at protein level). Expressed in squamous cell carcinoma, primary non-small cell lung cancer tumors and lung adenocarcinoma. [INDUCTION] Up-regulated by calcium in cells forming cell-cell contact sites. Up-regulated by DNA damaging agents like H(2)O(2) or ionizing radiation (IR).
Post-translational modifications : Phosphorylated by PLK1 in vitro. Hyperphosphorylated during the G2 phase of the cell cycle. Phosphorylation at Thr-373 occurs during the G2/M phase, relieves its auto-inhibition status and stimulates its GEF activity. Phosphorylation at Thr-444 in G2/M phase is required for subsequent binding with PLK1 and Rho exchange activation. Dephosphorylated at the time of cytokinesis. Phosphorylation at Thr-359 is required for its transformation activity in cancer cells.
Similarity : Contains 2 BRCT domains.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain. 
Database links : UniProtKB/Swiss-Prot: Q9H8V3.4
  磷酸化上皮细胞癌转化蛋白2抗体品牌储存温度：
 存在于血清、组织培养上清液或腹水中的抗体在-20℃下能够长期储存。低温存放不会损伤抗体活性。抗体的工作液通常储存于4℃中。此温度下，抗体的活性能稳定数月至数年亦不损失。抗体溶液不应反复冻融，因为反复冻融将导致部分抗体失活并产生不需要的蛋白—蛋白凝聚物。抗体的凝聚能导致活性丧失。这是由于抗原结合位点的空间构象被破坏，或由于产生的不溶物被离心或过滤而除去。冷凝蛋白类的抗体不能存放于4℃。因为在4℃下此类抗体会发生沉淀。一些鼠源性IgG3亚类的抗体有此特性。如果抗体在4℃下发生沉淀，就应该存放于室温下的叠氮钠溶液中。
 磷酸化上皮细胞癌转化蛋白2抗体品牌的分类：
按作用对象，可将其分为抗毒素、抗菌抗体、抗病毒抗体和亲细胞抗体（能与细胞结合的免疫球蛋白，如1型变态反应中的lgE反应素抗体，能吸附在靶细胞膜上）；按理化性质和生物学功能，可将其分为IgM、IgG、IgA、IgE、IgD五类；按抗体的来源，可 将其分为天然抗体和免疫抗体。抗体就是免疫球蛋白，是改变了的球蛋白分子。由特异性抗原刺激产生，抗体的产生是由于抗原侵入人体后引起各种免疫细胞相互作用，使淋巴细胞中的B细胞分化增殖而形成浆细胞，浆细胞可产生分泌抗体。
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Bolton Broth Supplement        
含225ml胰酪胨大豆多粘菌素肉汤均质袋     10个/包    用于蜡样芽孢杆菌的前增菌培养（GB 标准）
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含225ml生理盐水均质袋     10个/包    用于样品稀释处理
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品红亚硫酸钠琼脂（即用型）     100ml/瓶    用于饮用水，水源水中总大肠菌群的选择性分离和确证
Fuchsin Basic Sodium Sulfite Agar        
营养琼脂（即用型）     100ml/瓶    细菌计数、不含糖，可作血琼脂基础和传代用
Nutrient Agar        
0.1%蛋白胨水     500ml/瓶    用于样品的制备和稀释
0.1% Peptone Water        
PH7.0的NaCl蛋白胨缓冲液     500ml/瓶    用于制备药品样品的稀释液或冲洗液
PH7.0 NaCl-Peptone Buffer        
PH6.8磷酸盐缓冲液     500ml/瓶    用于样品稀释处理
PH 6.8 Phosphate Buffered Saline        
含青霉素酶TSA培养基平板（7cm）     10个/包    用于环境、器皿、设备和表面的无菌检测
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含青霉素酶接触皿培养基平板（6.5cm）     10个/包    用于环境、器皿、设备和表面的无菌检测
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RODAC接触皿（TSA）（6.5cm）     10个/包    用于环境、器皿、设备和表面的无菌检测
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自然杀伤细胞(NK)ELISA试剂盒    ，英文名：    NK ELISA Kit
自然抗性相关巨噬细胞蛋白2(NRAMP-2)ELISA试剂盒    ，英文名：    NRAMP-2 ELISA Kit
自然抗性相关巨噬细胞蛋白1(NRAMP-1)ELISA试剂盒    ，英文名：    NRAMP-1 ELISA Kit
桩蛋白(Pax)ELISA试剂盒    ，英文名：    Pax ELISA Kit
转移因子(TF)ELISA试剂盒    ，英文名：    TF ELISA Kit
转铁蛋白受体(TFR/CD71)ELISA试剂盒    ，英文名：    TFR/CD71 ELISA Kit
转录中介因子1-β(TRIM28/KAP1/RNF96/TIF1B)ELISA试剂盒    ，英文名：    TRIM28/KAP1/RNF96/TIF1B ELISA Kit
转录因子抗体-1(ATF-1)ELISA试剂盒    ，英文名：    ATF-1 ELISA Kit
转录因子SOX-5(SOX5)ELISA试剂盒    ，英文名：    SOX5 ELISA Kit
转录因子SOX-4(SOX4)ELISA试剂盒    ，英文名：    SOX4 ELISA Kit
转录因子1(AP-1)ELISA试剂盒    ，英文名：    AP-1 ELISA Kit
转甲状腺素蛋白(TTR)ELISA试剂盒    ，英文名：    TTR ELISA Kit
转化生长因子-β诱导蛋白IG-H3(TGFBI/BIGH3)ELISA试剂盒    ，英文名：    TGFBI/BIGH3 ELISA Kit
转化生长因子β受体3(TGFBR3)ELISA试剂盒    ，英文名：    TGFBR3 ELISA Kit
转化生长因子β受体2(TGF-βR2)ELISA试剂盒    ，英文名：    TGF-βR2 ELISA Kit
转化生长因子β受体2(TGFβR2)ELISA试剂盒    ，英文名：    TGFβR2 ELISA Kit
磷酸化上皮细胞癌转化蛋白2抗体品牌转化生长因子β受体1(TGFBR1)ELISA试剂盒    ，英文名：    TGFBR1 ELISA Kit
转化生长因子β3(TGF-β3)ELISA试剂盒    ，英文名：    TGF-β3 ELISA Kit
转化生长因子β2(TGFβ2)ELISA试剂盒    ，英文名：    TGFβ2 ELISA Kit
转化生长因子β1(TGF-β1)ELISA试剂盒    ，英文名：    TGF-β1 ELISA Kit
磷酸化上皮细胞癌转化蛋白2抗体品牌磷酸化上皮细胞癌转化蛋白2抗体品牌按理化性质和生物学功能，可将其分为IgM、IgG、IgA、IgE、IgD五类。
IgM抗体是免疫应答中首先分泌的抗体。它们在与抗原结合后启动补体的级联反应。它们还把入侵者相互连接起来，聚成一堆便于巨噬细胞的吞噬；
IgG抗体激活补体，中和多种毒素。IgG持续的时间长，是 唯一能在母亲妊娠期穿过胎盘保护胎儿的抗体。他们还从乳腺分泌进入初乳，使新生儿得 到保护；
IgA抗体进入身体的黏 膜表面，包括呼吸、消化、生殖等管道的黏膜，中和感染因子。还可以通过母乳的初乳把这种抗体输送到新生儿的消化道黏膜中，是在母乳中含量最多，最为重要的一类抗体；
IgE抗体的尾部与嗜碱细胞、肥大细胞的细胞膜结合。当抗体与抗原结合后，嗜碱细胞与肥大细胞释放组织胺一类物质促进炎症的发展。这也是引发速发型过敏反应的抗体；
IgD抗体的作用还不太清楚。它们主要出现在成熟的B淋巴细胞表面上，可能与B细胞的分化有关。（IgD于1995年从人骨髓瘤蛋白中发现，分子量为175kD，主要由扁桃体、脾等处浆细胞产生，人血清中IgD浓度为3～40μg/ml,不到血清总Ig的1%，在个体发育中合成较晚。IgD铰链区很长，且对蛋白酶水解敏感，因此IgD半衰期很短，仅2.8天。血清中IgD确切的免疫功能尚不清楚。在B细胞分化到成熟B细胞阶段，除了表达SmIgD，抗原刺激后表现为免疫耐受。成熟B细胞活化后或者活化后或者变成记忆B细胞时，SmIgD逐渐消失。