CHGA Antibody

CHGA Antibody

CATALOGUE NUMBER

ANT-351

SYNONYMS

CGA, CHGA, Vasostatin-2, Pituitary secretory protein I, SP-I.

TYPE

Mouse Anti Human Monoclonal.

INTRODUCTION

Chromgranin-A is part of the neuroendocrine secretory protein family. CHGA is located in secretory vesicles of neurons and endocrine cells. Chromgranin-A is a precursor to three biologically active peptides; vasostatin, pancreastatin, and parastatin. These peptides act as autocrine or paracrine negative modulators of the neuroendocrine system. Other peptides, including chromostatin, beta-granin, WE-14 and GE-25, are also derived from the full-length protein. Chromgranin-A has numerous biological activities on some tissues and organs and exerts a large spectrum of homeostatic actions, including antifungal and antimicrobial effect, modulation of cell adhesion, and inhibition of parathyroid hormone secretion.

CLONE

P3A5AT.

IMMUNOGEN

Anti-human CHGA mAb is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CHGA amino acids 19-131 purified from E. coli.

IG SUBCLASS

Mouse IgG1 heavy chain and κ light chain.

PURIFICATION METHOD

CHGA antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.

PHYSICAL APPEARANCE

Sterile Filtered colorless solution.

FORMULATION

1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.

STORAGE PROCEDURES

For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles.

STABILITY / SHELF LIFE

12 months at -20°C. 1 month at 4°C.

APPLICATIONS

CHGA antibody has been tested by ELISA, Western blot and immunohistochemistry analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot is 1:500 ~ 1:1,000 and immunohistochemistry analysis is 1:100~200. Recommended starting dilution for Western blot is 1:500 and Immunohistochemistry is 1:100.

USAGE

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.